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NQO1 Polyclonal Antibody

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NQO1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of MCF-7 cells, using NQO1 antibody 11451-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

NQO1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human pancreas cancer tissue slide using 11451-1-AP ( NQO1 Antibody) at dilution of 1:200 (under 40x lens).

NQO1 Antibody in Western Blot (WB) — MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 11451-1-AP (NQO1 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.

NQO1 Antibody in Flow Cytometry (Flow) — 1X10^6 MCF-7 cells were stained with 0.2ug NQO1 antibody (11451-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.

NQO1 Antibody in Immunoprecipitation (IP) — IP result of anti-NQO1 (IP:11451-1-AP, 4ug; Detection:11451-1-AP 1:300) with HepG2 cells lysate 3000 ug.

Product Details

11451-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	View 42 publications 42 publications
Immunohistochemistry (IHC)	-	View 3 publications 3 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:100-1:500	-
Immunocytochemistry (ICC/IF)	1:20-1:200	View 3 publications 3 publications
Flow Cytometry (Flow)	0.2 µg/1x10^6 cells	-
Immunoprecipitation (IP)	0.5-4.0 µg	-
Miscellaneous PubMed (Misc)	-	View 3 publications 3 publications

Product Specifications
Species Reactivity	Human
Published species	Human, Mouse, Pig, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	NQO1 Fusion Protein Ag2009 (1-274 aa encoded by BC007659) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.19 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

This antibody recognizes all the three isoforms (26-27 kDa and 31 kDa) of NQO1 and the homo-dimer form (66-70 kDa) of NQO1.

Immunogen sequence: MVGRRALIV LAHSERTSFN YAMKEAAAAA LKKKGWEVVE SDLYAMNFNP IISRKDITGK LKDPANFQYP AESVLAYKEG HLSPDIVAEQ KKLEAADLVI FQFPLQWFGV PAILKGWFER VFIGEFAYTY AAMYDKGPFR SKKAVLSITT GGSGSMYSLQ GIHGDMNVIL WPIQSGILHF CGFQVLEPQL TYSIGHTPAD ARIQILEGWK KRLENIWDET PLYFAPSSLF DLNFQAGFLM KKEVQDEEKN KKFGLSVGHH LGKSIPTDNQ IKARK (1-274 aa encoded by BC007659)

Target Information

NQO1 is a member of the NAD(P)H dehydrogenase (quinone) family and a cytoplasmic 2-electron reductase. This FAD-binding protein forms homodimers and reduces quinones to hydroquinones. NQO1's enzymatic activity prevents the one electron reduction of quinones that results in the production of radical species. Altered expression of the protein has been seen in many tumors and is also associated with Alzheimer's disease (AD).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Azoreductase; diaphorase (NADH/NADPH) (cytochrome b-5 reductase); Diaphorase 4; diaphorase-4; dioxin-inducible 1; DT-diaphorase; DTD; Menadione reductase; NAD(P)H dehydrogenase [quinone] 1; NAD(P)H dehydrogenase, quinone 1; NAD(P)H:menadione oxidoreductase 1; NAD(P)H:Quinone acceptor oxidoreductase type 1; NAD(P)H:quinone oxidoreductase 1; NAD(P)H:quinone oxireductase; NQ01; Phylloquinone reductase; QR1; Quinone reductase 1

Gene Aliases: DHQU; DIA4; DTD; NMOR1; NMORI; NQO1; QR1

UniProt ID: (Human) P15559

Entrez Gene ID: (Human) 1728

Molecular Function: oxidoreductase

Function(s)

regulation of cellular amino acid metabolic process xenobiotic metabolic process nitric oxide biosynthetic process synaptic transmission, cholinergic aging response to nutrient response to toxic substance removal of superoxide radicals response to estradiol negative regulation of catalytic activity positive regulation of neuron apoptotic process response to ethanol oxidation-reduction process

Process(es)

NAD(P)H dehydrogenase (quinone) activity cytochrome-b5 reductase activity, acting on NAD(P)H superoxide dismutase activity protein binding identical protein binding poly(A) RNA binding

NQO1 Polyclonal Antibody

Product Details

11451-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Product Specific Information

Target Information

Bioinformatics

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