Proteintech

NR1D1 Polyclonal Antibody

Product Image Gallery

NR1D1 Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of ( 10% Formaldehyde ) fixed NIH/3T3 cells using 14506-1-AP (NR1D1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

NR1D1 Antibody in Western Blot (WB) — NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14506-1-AP ( NR1D1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

NR1D1 Antibody in Immunoprecipitation (IP) — IP result of anti-NR1D1 (IP:14506-1-AP, 4ug; Detection:14506-1-AP 1:300) with A549 cells lysate 1200ug.

Product Details

Tested Applications

Dilution

ChIP assay (ChIP)

Assay-dependent

Immunocytochemistry (ICC)

1:50-1:500

Immunofluorescence (IF)

1:50-1:500

Immunoprecipitation (IP)

0.5-4.0 µg

Western Blot (WB)

1:500-1:2000

Published Applications

Immunofluorescence (IF)

See 2 publications below

Western Blot (WB)

See 2 publications below

ChIP assay (ChIP)

See 1 publication below

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

NR1D1 Fusion Protein Ag5964

Conjugate

Unconjugated

Form

Liquid

Concentration

0.26 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.1% sodium azide

Storage conditions

-20°C

Product Specific Information

This is a rabbit polyclonal antibody raised against the N terminus of human NR1D1.

Target Information

This gene encodes a member of the nuclear hormone receptor family, specifically the NR1 subfamily of receptors. The encoded protein functions as a transcriptional repressor and may play a role in circadian rhythms and carbohydrate and lipid metabolism. Alternatively spliced transcript variants have been described.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: ear 1; EAR-1; EAR1; hRev; NR1D1; nuclear receptor protein; nuclear receptor Rev-ErbA-alpha; Nuclear receptor subfamily 1 group D member 1; nuclear receptor subfamily 1, group D, member 1; orphan nuclear receptor; Rev erbA alpha; rev-erb alpha; rev-erbA alpha; rev-erbA(alpha); Rev-erbA-alpha; Rev-ErbA-alpha protein; Rev-ErbAalpha; REV-ERBalpha; Reverb alpha; THRA1; THRAL; V erbA related protein 1; V-erbA-related protein 1

Gene Aliases: A530070C09Rik; ear-1; EAR1; HREV; NR1D1; R75201; THRA1; THRAL

UniProt ID: (Human) P20393, (Mouse) Q3UV55

Entrez Gene ID: (Human) 9572, (Mouse) 217166

Molecular Function: nuclear hormone receptor nucleic acid binding receptor transcription factor