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NR1D2 Antibody (CF806588) in WB
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NR1D2 (RC206338, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-NR1D2. Positive lysates LY417483 (100 µg) and LC417483 (20 µg) can be purchased separately from OriGene.
Product Details
CF806588
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
OTI3D4
Immunogen
Human recombinant protein fragment corresponding to amino acids 1-294 of human NR1D2 produced in E.coli.
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 8% trehalose
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
Target Information
This gene encodes a member of the nuclear hormone receptor family, specifically the NR1 subfamily of receptors. The encoded protein functions as a transcriptional repressor and may play a role in circadian rhythms and carbohydrate and lipid metabolism. Alternatively spliced transcript variants have been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: EAR-1R; HZF2; nuclear receptor Rev-ErbA beta variant 1; nuclear receptor Rev-ErbA beta variant 2; Nuclear receptor subfamily 1 group D member 2; nuclear receptor subfamily 1, group D, member 2; Orphan nuclear hormone receptor BD73; Rev-erb alpha-related receptor; Rev-erb-beta; Rev-erba beta; rev-erba-alpha-related receptor; Rev-erbabeta; RVR; V-erbA-related protein 1-related
Gene Aliases: BD73; EAR-1R; NR1D2; RVR
UniProt ID: (Human) Q14995
Entrez Gene ID: (Human) 9975
core promoter sequence-specific DNA binding
steroid hormone receptor activity
RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding
protein binding
zinc ion binding
C4 zinc finger nuclear receptor
DNA-binding transcription factor
zinc finger transcription factor
gene-specific transcriptional regulator
regulation of transcription, DNA-templated
transcription initiation from RNA polymerase II promoter
regulation of lipid metabolic process
intracellular receptor signaling pathway
regulation of circadian rhythm
steroid hormone mediated signaling pathway
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
rhythmic process
regulation of inflammatory response
lipid homeostasis
regulation of energy homeostasis
regulation of skeletal muscle cell differentiation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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