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Proteintech

NR1H4 Polyclonal Antibody

2 References
View all (15) FXR antibodies
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Product Details

25055-1-AP

Applications Tested Dilution Publications

Western Blot (WB)

 1:500-1:2,000 View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

 Assay-dependent View 1 publication 1 publication
Product Specifications

Species Reactivity

 Human

Published species

 Rat

Host/Isotype

 Rabbit / IgG

Class

 Polyclonal

Type

 Antibody

Immunogen

NR1H4 Fusion Protein Ag21878 (1-121 aa encoded by BC130573)
View antigen

Conjugate

 Unconjugated

Form

 Liquid

Concentration

 0.27 mg/mL

Purification

 Antigen affinity chromatography

Storage buffer

 PBS, pH 7.3, with 50% glycerol

Contains

 0.1% sodium azide

Storage conditions

 -20°C

Product Specific Information

Immunogen sequence: MGSKMNLIE HSHLPTTDEF SFSENLFGVL TEQVAGPLGQ NLEVEPYSQY SNVQFPQVQP QISSSSYYSN LGFYPQQPEE WYSPGIYELR RMPAETLYQG ETEVAEMPVT KKPRMGASAG RI (1-121 aa encoded by BC130573)

Target Information

FXR (farnesoid X receptor; NR1H4) belongs to nuclear receptor superfamily of ligand-activated transcription factors (NR1 subfamily) and acts as a receptor for bile acids such as chenodeoxycholic acid, lithocholic acid and deoxycholic acid. FXR plays an essential role in bile acid homeostasis through the regulation of genes involved in bile acid synthesis, conjugation and enterohepatic circulation. FXR also regulates lipid and glucose homeostasis and is involved innate immune response. The FXR-RXR heterodimer binds predominantly to farnesoid X receptor response elements (FXREs) containing two inverted repeats of the consensus sequence 5'-AGGTCA-3' in which the monomers are spaced by 1 nucleotide (IR-1) but also to tandem repeat DR1 sites with lower affinity, and can be activated by either FXR or RXR-specific ligands. In the liver FXR activates transcription of the corepressor NR0B2 thereby indirectly inhibiting CYP7A1 and CYP8B1 (involved in BA synthesis) implicating at least in part histone demethylase KDM1A resulting in epigenomic repression, and SLC10A1/NTCP (involved in hepatic uptake of conjugated BAs). FXR activates transcription of SLC27A5/BACS and BAAT (involved in BA conjugation), ABCB11/BSEP (involved in bile salt export) by directly recruiting histone methyltransferase CARM1, and ABCC2/MRP2 (involved in secretion of conjugated BAs) and ABCB4 (involved in secretion of phosphatidylcholine in the small intestine). (From Uniprot)

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Bile acid receptor; farnesoid X nuclear receptor; Farnesoid X-activated receptor; Farnesol receptor HRR-1; MGC163445; Nuclear receptor subfamily 1 group H member 4; Retinoid X receptor-interacting protein 14; RXR-interacting protein 14

Gene Aliases: BAR; FXR; HRR-1; HRR1; NR1H4; RIP14

UniProt ID: (Human) Q96RI1

Entrez Gene ID: (Human) 9971

Function(s)
negative regulation of transcription from RNA polymerase II promoter nitrogen catabolite activation of transcription from RNA polymerase II promoter regulation of carbohydrate metabolic process transcription initiation from RNA polymerase II promoter inflammatory response signal transduction Notch signaling pathway bile acid metabolic process response to glucose regulation of low-density lipoprotein particle clearance bile acid and bile salt transport intracellular receptor signaling pathway response to lipopolysaccharide toll-like receptor 4 signaling pathway regulation of urea metabolic process histone H3-R17 methylation cellular triglyceride homeostasis bile acid signaling pathway intracellular bile acid receptor signaling pathway negative regulation of apoptotic process negative regulation of I-kappaB kinase/NF-kappaB signaling steroid hormone mediated signaling pathway innate immune response positive regulation of transcription from RNA polymerase II promoter digestive tract development regulation of insulin secretion involved in cellular response to glucose stimulus regulation of bile acid biosynthetic process negative regulation of bile acid biosynthetic process cellular response to fatty acid cellular response to organonitrogen compound interleukin-17 secretion regulation of cholesterol metabolic process negative regulation of interferon-gamma secretion negative regulation of tumor necrosis factor secretion positive regulation of glutamate metabolic process positive regulation of ammonia assimilation cycle
Process(es)
RNA polymerase II distal enhancer sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional activator activity, RNA polymerase II transcription factor binding transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding steroid hormone receptor activity transcription coactivator activity transcription corepressor activity RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding thyroid hormone receptor activity protein binding zinc ion binding ligand-dependent nuclear receptor binding bile acid binding bile acid receptor activity peptide binding sequence-specific DNA binding retinoid X receptor binding chenodeoxycholic acid binding

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