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NR1H4 Polyclonal Antibody

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NR1H4 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using (Product # 25055-1-AP) (NR1H4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

NR1H4 Antibody in Western Blot (WB) — MKN-45 cells were subjected to SDS PAGE followed by western blot with 25055-1-AP ( NR1H4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Product Details

25055-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 28 publications 28 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200

Immunocytochemistry (ICC/IF)

Assay-dependent

View 8 publications 8 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

NR1H4 Fusion Protein Ag21878 (1-121 aa encoded by BC130573)

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.27 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Product Specific Information

Immunogen sequence: MGSKMNLIE HSHLPTTDEF SFSENLFGVL TEQVAGPLGQ NLEVEPYSQY SNVQFPQVQP QISSSSYYSN LGFYPQQPEE WYSPGIYELR RMPAETLYQG ETEVAEMPVT KKPRMGASAG RI (1-121 aa encoded by BC130573)

Target Information

FXR (farnesoid X receptor; NR1H4) belongs to nuclear receptor superfamily of ligand-activated transcription factors (NR1 subfamily) and acts as a receptor for bile acids such as chenodeoxycholic acid, lithocholic acid and deoxycholic acid. FXR plays an essential role in bile acid homeostasis through the regulation of genes involved in bile acid synthesis, conjugation and enterohepatic circulation. FXR also regulates lipid and glucose homeostasis and is involved innate immune response. The FXR-RXR heterodimer binds predominantly to farnesoid X receptor response elements (FXREs) containing two inverted repeats of the consensus sequence 5'-AGGTCA-3' in which the monomers are spaced by 1 nucleotide (IR-1) but also to tandem repeat DR1 sites with lower affinity, and can be activated by either FXR or RXR-specific ligands. In the liver FXR activates transcription of the corepressor NR0B2 thereby indirectly inhibiting CYP7A1 and CYP8B1 (involved in BA synthesis) implicating at least in part histone demethylase KDM1A resulting in epigenomic repression, and SLC10A1/NTCP (involved in hepatic uptake of conjugated BAs). FXR activates transcription of SLC27A5/BACS and BAAT (involved in BA conjugation), ABCB11/BSEP (involved in bile salt export) by directly recruiting histone methyltransferase CARM1, and ABCC2/MRP2 (involved in secretion of conjugated BAs) and ABCB4 (involved in secretion of phosphatidylcholine in the small intestine). (From Uniprot)

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Bisphenol A induced hepatic steatosis by disturbing bile acid metabolism and FXR/TGR5 signaling pathways via remodeling the gut microbiota in CD-1 mice.

The Science of the total environment

Hong T,Zou J,He Y,Zhang H,Liu H,Mai H,Yang J,Cao Z,Chen X,Yao J,Feng D

Fri Sep 01 00:00:00 EDT 2023

Bisphenol A induced hepatic steatosis by disturbing bile acid metabolism and FXR/TGR5 signaling pathways via remodeling the gut microbiota in CD-1 mice.

The Science of the total environment

Hong T,Zou J,He Y,Zhang H,Liu H,Mai H,Yang J,Cao Z,Chen X,Yao J,Feng D

Fri Sep 01 00:00:00 EDT 2023

Wen-Shen-Jian-Pi-Hua-Tan decoction protects against early obesity-related glomerulopathy by improving renal bile acid composition and suppressing lipogenesis, inflammation, and fibrosis.

Phytomedicine : international journal of phytotherapy and phytopharmacology

Song D,Zhang A,Hu X,Zeng M,Zhou H

Tue Jul 25 00:00:00 EDT 2023

Wen-Shen-Jian-Pi-Hua-Tan decoction protects against early obesity-related glomerulopathy by improving renal bile acid composition and suppressing lipogenesis, inflammation, and fibrosis.

Phytomedicine : international journal of phytotherapy and phytopharmacology

Song D,Zhang A,Hu X,Zeng M,Zhou H

Tue Jul 25 00:00:00 EDT 2023

The choleretic role of tauroursodeoxycholic acid exacerbates alpha-naphthylisothiocyanate induced cholestatic liver injury through the FXR/BSEP pathway.

Journal of applied toxicology : JAT

Zhao J,Song G,Weng F,Li Y,Zou B,Jin J,Yan D,Sun X,Liu C,Qiu FR

Sat Jul 01 00:00:00 EDT 2023

Regulon analysis identifies protective FXR and CREB5 in proximal tubules in early diabetic kidney disease.

BMC nephrology

Shi W,Le W,Tang Q,Shi S,Shi J

Mon Jun 19 00:00:00 EDT 2023

Regulon analysis identifies protective FXR and CREB5 in proximal tubules in early diabetic kidney disease.

BMC nephrology

Shi W,Le W,Tang Q,Shi S,Shi J

Mon Jun 19 00:00:00 EDT 2023

Bacteroides dorei BDX-01 alleviates DSS-induced experimental colitis in mice by regulating intestinal bile salt hydrolase activity and the FXR-NLRP3 signaling pathway.

Frontiers in pharmacology

Sun X,Chen Z,Yu L,Zeng W,Sun B,Fan H,Bai Y

Sun Jun 11 00:00:00 EDT 2023

Dietary Isoquercetin Reduces Hepatic Cholesterol and Triglyceride in NAFLD Mice by Modulating Bile Acid Metabolism via Intestinal FXR-FGF15 Signaling.

Journal of agricultural and food chemistry

Zhang C,Shi Z,Lei H,Wu F,Chen C,Cao Z,Song Y,Zhang C,Zhou J,Lu Y,Zhang L

Wed May 24 00:00:00 EDT 2023

Jiang-Tang-San-Huang pill alleviates type 2 diabetes mellitus through modulating the gut microbiota and bile acids metabolism.

Phytomedicine : international journal of phytotherapy and phytopharmacology

Tawulie D,Jin L,Shang X,Li Y,Sun L,Xie H,Zhao J,Liao J,Zhu Z,Cui H,Wen W

Mon May 01 00:00:00 EDT 2023

Polygoni Multiflori Radix interferes with bile acid metabolism homeostasis by inhibiting Fxr transcription, leading to cholestasis.

Frontiers in pharmacology

Dai Y,Jia Z,Fang C,Zhu M,Yan X,Zhang Y,Wu H,Feng M,Liu L,Huang B,Li Y,Liu J,Xiao H

Fri Mar 24 00:00:00 EDT 2023

Supplemental Clostridium butyricum modulates lipid metabolism by reshaping the gut microbiota composition and bile acid profile in IUGR suckling piglets.

Journal of animal science and biotechnology

Zhang X,Yun Y,Lai Z,Ji S,Yu G,Xie Z,Zhang H,Zhong X,Wang T,Zhang L

Mon Mar 13 00:00:00 EDT 2023

Gamma-Muricholic Acid Inhibits Nonalcoholic Steatohepatitis: Abolishment of Steatosis-Dependent Peroxidative Impairment by FXR/SHP/LXRα/FASN Signaling.

Nutrients

Xie Y,Shen F,He Y,Guo C,Yang R,Cao H,Pan Q,Fan J

Thu Mar 02 00:00:00 EST 2023

Taurocholic Acid and Glycocholic Acid Inhibit Inflammation and Activate Farnesoid X Receptor Expression in LPS-Stimulated Zebrafish and Macrophages.

Molecules (Basel, Switzerland)

Ge X,Huang S,Ren C,Zhao L

Tue Feb 21 00:00:00 EST 2023

Sodium cholate ameliorates nonalcoholic steatohepatitis by activation of FXR signaling.

Hepatology communications

Pan L,Yu Z,Liang X,Yao J,Fu Y,He X,Ren X,Chen J,Li X,Lu M,Lan T

Wed Feb 01 00:00:00 EST 2023

Resveratrol Modulates the Redox Response and Bile Acid Metabolism to Maintain the Cholesterol Homeostasis in Fish Megalobrama amblycephala Offered a High-Carbohydrate Diet.

Antioxidants (Basel, Switzerland)

Ge Y,Zhang L,Chen W,Sun M,Liu W,Li X

Tue Jan 03 00:00:00 EST 2023

Impaired FXR-CPT1a signaling contributes to parenteral nutrition-induced villus atrophy in short-bowel syndrome.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Zhao Y,Feng H,Wang Y,Jiang L,Yan J,Cai W

Sun Jan 01 00:00:00 EST 2023

Impaired FXR-CPT1a signaling contributes to parenteral nutrition-induced villus atrophy in short-bowel syndrome.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Zhao Y,Feng H,Wang Y,Jiang L,Yan J,Cai W

Sun Jan 01 00:00:00 EST 2023

The protective effects of sulforaphane on high-fat diet-induced metabolic associated fatty liver disease in mice via mediating the FXR/LXRα pathway.

Food & function

Ma S,Pang X,Tian S,Sun J,Hu Q,Li X,Lu Y

Tue Dec 13 00:00:00 EST 2022

Validation of MAPK signalling pathway as a key role of paeoniflorin in the treatment of intrahepatic cholestasis of pregnancy based on network pharmacology and metabolomics.

European journal of pharmacology

Wang J,Wen J,Ma X,Yang J,Zhang Z,Xie S,Wei S,Jing M,Li H,Lang L,Zhou X,Zhao Y

Tue Nov 15 00:00:00 EST 2022

Ginsenoside Rc, as an FXR activator, alleviates acetaminophen-induced hepatotoxicity via relieving inflammation and oxidative stress.

Frontiers in pharmacology

Zhong Y,Chen Y,Pan Z,Tang K,Zhong G,Guo J,Cui T,Li T,Duan S,Yang X,Gao Y,Wang Q,Zhang D

Tue Oct 25 00:00:00 EDT 2022

Theacrine ameliorates experimental liver fibrosis in rats by lowering cholesterol storage via activation of the Sirtuin 3-farnesoid X receptor signaling pathway.

Chemico-biological interactions

Lv XT,Wang RH,Liu XT,Ye YJ,Liu XY,Qiao JD,Wang GE

Thu Sep 01 00:00:00 EDT 2022

Theacrine ameliorates experimental liver fibrosis in rats by lowering cholesterol storage via activation of the Sirtuin 3-farnesoid X receptor signaling pathway.

Chemico-biological interactions

Lv XT,Wang RH,Liu XT,Ye YJ,Liu XY,Qiao JD,Wang GE

Thu Sep 01 00:00:00 EDT 2022

Mesenchymal stem cell-derived exosome mitigates colitis via the modulation of the gut metagenomics-metabolomics-farnesoid X receptor axis.

Biomaterials science

Ocansey DKW,Zhang Z,Xu X,Liu L,Amoah S,Chen X,Wang B,Zhang X,Mao F

Wed Aug 24 00:00:00 EDT 2022

Mesenchymal stem cell-derived exosome mitigates colitis via the modulation of the gut metagenomics-metabolomics-farnesoid X receptor axis.

Biomaterials science

Ocansey DKW,Zhang Z,Xu X,Liu L,Amoah S,Chen X,Wang B,Zhang X,Mao F

Wed Aug 24 00:00:00 EDT 2022

Mesenchymal stem cell-derived exosome mitigates colitis via the modulation of the gut metagenomics-metabolomics-farnesoid X receptor axis.

Biomaterials science

Ocansey DKW,Zhang Z,Xu X,Liu L,Amoah S,Chen X,Wang B,Zhang X,Mao F

Wed Aug 24 00:00:00 EDT 2022

Mesenchymal stem cell-derived exosome mitigates colitis via the modulation of the gut metagenomics-metabolomics-farnesoid X receptor axis.

Biomaterials science

Ocansey DKW,Zhang Z,Xu X,Liu L,Amoah S,Chen X,Wang B,Zhang X,Mao F

Wed Aug 24 00:00:00 EDT 2022

Alpha-linolenic acid inhibits hepatocellular carcinoma cell growth through Farnesoid X receptor/β-catenin signaling pathway.

Nutrition & metabolism

Feng S,Xie X,Chen C,Zuo S,Zhao X,Li H

Tue Aug 23 00:00:00 EDT 2022

FXR deficiency in hepatocytes disrupts the bile acid homeostasis and inhibits autophagy to promote liver injury in Schistosoma japonicum-infected mice.

PLoS neglected tropical diseases

Zhang B,Li J,Zong X,Wang J,Xin L,Song H,Zhang W,Koda S,Hua H,Zhang B,Yu Q,Zheng KY,Yan C

Mon Aug 01 00:00:00 EDT 2022

Gut microbiota mediates methamphetamine-induced hepatic inflammation via the impairment of bile acid homeostasis.

Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association

Zhang KK,Liu JL,Chen LJ,Li JH,Yang JZ,Xu LL,Chen YK,Zhang QY,Li XW,Liu Y,Zhao D,Xie XL,Wang Q

Mon Aug 01 00:00:00 EDT 2022

Lactobacillus casei SYF-08 Protects Against Pb-Induced Injury in Young Mice by Regulating Bile Acid Metabolism and Increasing Pb Excretion.

Frontiers in nutrition

Chen Z,Tang Z,Kong J,Chen L,Liu J,Li Y,Huang W,Li W,Wu J,Zhao W,Meng X,Fan H

Tue Jul 19 00:00:00 EDT 2022

Hepatoprotective Effects of Glycyrrhetinic Acid on Lithocholic Acid-Induced Cholestatic Liver Injury Through Choleretic and Anti-Inflammatory Mechanisms.

Frontiers in pharmacology

Wang Q,Song GC,Weng FY,Zou B,Jin JY,Yan DM,Tan B,Zhao J,Li Y,Qiu FR

Sat Jul 16 00:00:00 EDT 2022

DMRT2 Interacts With FXR and Improves Insulin Resistance in Adipocytes and a Mouse Model.

Frontiers in endocrinology

Tao J,Yu XL,Yuan YJ,Shen X,Liu J,Gu PP,Wang Z,Ma YT,Li GQ

Fri Apr 22 00:00:00 EDT 2022

DMRT2 Interacts With FXR and Improves Insulin Resistance in Adipocytes and a Mouse Model.

Frontiers in endocrinology

Tao J,Yu XL,Yuan YJ,Shen X,Liu J,Gu PP,Wang Z,Ma YT,Li GQ

Fri Apr 22 00:00:00 EDT 2022

Lignans from the seeds of Herpetospermum pedunculosum and their farnesoid X receptor-activating effect.

Phytochemistry

Meng F,Ma Y,Zhan H,Zong W,Linghu L,Wang Z,Lan X,Liao Z,Chen M

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Probiotic-fermented black tartary buckwheat alleviates hyperlipidemia and gut microbiota dysbiosis in rats fed with a high-fat diet.

Food & function

Ren Y,Wu S,Xia Y,Huang J,Ye J,Xuan Z,Li P,Du B

Mon Jul 05 00:00:00 EDT 2021

Dolomiaea souliei ethyl acetate extract protected against α-naphthylisothiocyanate-induced acute intrahepatic cholestasis through regulation of farnesoid x receptor-mediated bile acid metabolism.

Phytomedicine : international journal of phytotherapy and phytopharmacology

Meng F,Zong W,Wei X,Tao Y,Wang G,Liao Z,Chen M

Thu Jul 01 00:00:00 EDT 2021

Picroside II alleviates liver injury induced by alpha-naphthylisothiocyanate through AMPK-FXR pathway.

Toxicology and applied pharmacology

Li T,Zheng R,Xu L,Zhou M,Wang X,Guo Q,Ji H,Li L

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The nuclear translocation of transketolase inhibits the farnesoid receptor expression by promoting the binding of HDAC3 to FXR promoter in hepatocellular carcinoma cell lines.

Cell death & disease

Li M,Zhang X,Lu Y,Meng S,Quan H,Hou P,Tong P,Chai D,Gao X,Zheng J,Tong X,Bai J

Thu Jan 16 00:00:00 EST 2020

Protective effect of dioscin against thioacetamide-induced acute liver injury via FXR/AMPK signaling pathway in vivo.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Zheng L,Yin L,Xu L,Qi Y,Li H,Xu Y,Han X,Liu K,Peng J

Mon Jan 01 00:00:00 EST 2018

Protective effect of dioscin against thioacetamide-induced acute liver injury via FXR/AMPK signaling pathway in vivo.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Zheng L,Yin L,Xu L,Qi Y,Li H,Xu Y,Han X,Liu K,Peng J

Mon Jan 01 00:00:00 EST 2018

Protective effect of dioscin against thioacetamide-induced acute liver injury via FXR/AMPK signaling pathway in vivo.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Zheng L,Yin L,Xu L,Qi Y,Li H,Xu Y,Han X,Liu K,Peng J

Mon Jan 01 00:00:00 EST 2018

Protective effect of dioscin against thioacetamide-induced acute liver injury via FXR/AMPK signaling pathway in vivo.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Zheng L,Yin L,Xu L,Qi Y,Li H,Xu Y,Han X,Liu K,Peng J

Mon Jan 01 00:00:00 EST 2018

Bioinformatics

Protein Aliases: Bile acid receptor; farnesoid X nuclear receptor; Farnesoid X-activated receptor; Farnesol receptor HRR-1; MGC163445; Nuclear receptor subfamily 1 group H member 4; Retinoid X receptor-interacting protein 14; RXR-interacting protein 14

Gene Aliases: BAR; FXR; HRR-1; HRR1; NR1H4; RIP14

UniProt ID: (Human) Q96RI1

Entrez Gene ID: (Human) 9971

Function(s)

negative regulation of transcription from RNA polymerase II promoter nitrogen catabolite activation of transcription from RNA polymerase II promoter regulation of carbohydrate metabolic process transcription initiation from RNA polymerase II promoter inflammatory response signal transduction Notch signaling pathway bile acid metabolic process response to glucose regulation of low-density lipoprotein particle clearance bile acid and bile salt transport intracellular receptor signaling pathway response to lipopolysaccharide toll-like receptor 4 signaling pathway regulation of urea metabolic process histone H3-R17 methylation cellular triglyceride homeostasis bile acid signaling pathway intracellular bile acid receptor signaling pathway negative regulation of apoptotic process negative regulation of I-kappaB kinase/NF-kappaB signaling steroid hormone mediated signaling pathway innate immune response positive regulation of transcription from RNA polymerase II promoter digestive tract development regulation of insulin secretion involved in cellular response to glucose stimulus regulation of bile acid biosynthetic process negative regulation of bile acid biosynthetic process cellular response to fatty acid cellular response to organonitrogen compound interleukin-17 secretion regulation of cholesterol metabolic process negative regulation of interferon-gamma secretion negative regulation of tumor necrosis factor secretion positive regulation of glutamate metabolic process positive regulation of ammonia assimilation cycle

Process(es)

RNA polymerase II distal enhancer sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional activator activity, RNA polymerase II transcription factor binding transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding steroid hormone receptor activity transcription coactivator activity transcription corepressor activity RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding thyroid hormone receptor activity protein binding zinc ion binding ligand-dependent nuclear receptor binding bile acid binding bile acid receptor activity peptide binding sequence-specific DNA binding retinoid X receptor binding chenodeoxycholic acid binding

NR1H4 Polyclonal Antibody

Product Details

25055-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Product Specific Information

Target Information

Bioinformatics

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