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Invitrogen
NSD1 Polyclonal Antibody
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FIGURE: 1 / 6
NSD1 Antibody (PA5-50857) in ICC/IF
Immunofluorescence analysis of NSD1 was performed using 70% confluent log phase HEK-293 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with NSD1 Polyclonal Antibody (Product # PA5-50857) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790)... View More
Product Details
PA5-50857
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
C. elegans,
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide of human nuclear receptor binding SET domain protein 1
Conjugate
Form
Liquid
Concentration
1.5 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 40% glycerol
Contains
0.05% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
The antibody detects endogenous levels of total NSD1 / KMT3B protein.
Target Information
This gene encodes a protein containing a SET domain, 2 LXXLL motifs, 3 nuclear translocation signals (NLSs), 4 plant homeodomain (PHD) finger regions, and a proline-rich region. The encoded protein enhances androgen receptor (AR) transactivation, and this enhancement can be increased further in the presence of other androgen receptor associated coregulators. This protein may act as a nucleus-localized, basic transcriptional factor and also as a bifunctional transcriptional regulator. Mutations of this gene have been associated with Sotos syndrome and Weaver syndrome. One version of childhood acute myeloid leukemia is the result of a cryptic translocation with the breakpoints occurring within nuclear receptor-binding Su-var, enhancer of zeste, and trithorax domain protein 1 on chromosome 5 and nucleoporin, 98-kd on chromosome 11. Two transcript variants encoding distinct isoforms have been identified for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Androgen receptor coactivator 267 kDa protein; androgen receptor-associated coregulator 267; Androgen receptor-associated protein of 267 kDa; H3-K36-HMTase; H4-K20-HMTase; Histone-lysine N-methyltransferase, H3 lysine-36 specific; Lysine N-methyltransferase 3B; NR-binding SET domain-containing protein; Nuclear receptor-binding SET domain-containing protein 1
Gene Aliases: ARA267; KMT3B; NSD1; SOTOS; SOTOS1; STO
UniProt ID: (Human) Q96L73
Entrez Gene ID: (Human) 64324
RNA polymerase II core promoter sequence-specific DNA binding
chromatin binding
transcription cofactor activity
transcription corepressor activity
zinc ion binding
histone-lysine N-methyltransferase activity
estrogen receptor binding
histone methyltransferase activity (H4-K20 specific)
retinoic acid receptor binding
retinoid X receptor binding
thyroid hormone receptor binding
histone methyltransferase activity (H3-K36 specific)
androgen receptor binding
negative regulation of transcription from RNA polymerase II promoter
regulation of histone H3-K36 methylation
transcription, DNA-templated
histone H3-K36 methylation
histone methylation
regulation of peptidyl-serine phosphorylation
histone H4-K20 methylation
positive regulation of transcription, DNA-templated
regulation of RNA polymerase II regulatory region sequence-specific DNA binding
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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