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          • Primary Antibodies ›
          • NUMB Antibodies

          Invitrogen

          NUMB Polyclonal Antibody

          View all (65) NUMB antibodies
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          Cite NUMB Polyclonal Antibody

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          • Antibody Testing Data (5)
          NUMB Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 5

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          NUMB Antibody (PA5-21143) in WB

          Western blot was performed using Anti-NUMB Polyclonal Antibody (Product # PA5-21143) on whole cell extracts (30 µg lysate) of U-87 MG (Lane1), PC-3 (Lane 2), DU 145 (Lane 3), A549 (Lane 4), SH-SY5Y(Lane 5), HeLa (Lane 6), Mouse Lungs (Lane 7), Mouse Colon (Lane 8), Mouse Heart (Lane 9) and Mouse Kidney (Lane 10)and NUMB major isoform 65 kDa was observed along with other isoforms between 53-70 kDa in all samples except Mouse Colon, Heart and Kidney. Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) b... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          Product Details

          PA5-21143

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          0.25-0.5 µg/mL
          -

          Immunohistochemistry (IHC)

          5 µg/mL
          -

          Immunocytochemistry (ICC/IF)

          5-20 µg/mL
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          A 17 amino acid peptide near the carboxy terminus of human NUMB.
          View immunogen

          Conjugate

          Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS

          Contains

          0.02% sodium azide

          Storage conditions

          Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C

          RRID

          AB_11154464

          Product Specific Information

          A suggested positive control is mouse lung tissue lysate.

          PA5-21143 can be used with blocking peptide PEP-1257.

          Target Information

          Protein NUMB homolog is an evolutionarily conserved protein that controls the differentiation of neuronal progenitor cells. Recent studies have revealed that NUMB functions in clathrin-dependent endocytosis by binding to the AP-2 complex. NUMB is also expressed in postmitotic neurons and plays a role in axonal growth. Neural cells expressing Numb isoforms with short phosphotyrosine-binding (SPTB) domain undergo extensive neurite outgrowth, an effect that can be blocked by voltage-gated Ca2+ channel (VGCC) inhibitor or by Ca2+ chelator.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: h-Numb; m-Nb; m-numb; mnb; numb gene homolog; numb homolog; Protein numb homolog; Protein S171

          View more View less

          Gene Aliases: c14_5527; C14orf41; Nb; NUMB; S171

          View more View less

          UniProt ID: (Human) P49757, (Mouse) Q9QZS3

          View more View less

          Entrez Gene ID: (Human) 8650, (Mouse) 18222, (Rat) 29419

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          Molecular Function: scaffold/adaptor protein

          Function(s)
          axonogenesis lateral ventricle development neuroblast division in subventricular zone positive regulation of cell migration positive regulation of polarized epithelial cell differentiation adherens junction organization negative regulation of Notch signaling pathway positive regulation of neurogenesis lung epithelial cell differentiation cell-cell adhesion negative regulation of protein localization to plasma membrane multicellular organism development nervous system development neuroblast proliferation forebrain development regulation of neuron differentiation positive regulation of dendrite morphogenesis cell proliferation cell differentiation
          Process(es)
          protein binding beta-catenin binding alpha-catenin binding cadherin binding involved in cell-cell adhesion cadherin binding
          It has to be done as per old AB suggested Products section.
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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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