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NUMB Antibody (PA5-21143) in WB
Western blot was performed using Anti-NUMB Polyclonal Antibody (Product # PA5-21143) on whole cell extracts (30 µg lysate) of U-87 MG (Lane1), PC-3 (Lane 2), DU 145 (Lane 3), A549 (Lane 4), SH-SY5Y(Lane 5), HeLa (Lane 6), Mouse Lungs (Lane 7), Mouse Colon (Lane 8), Mouse Heart (Lane 9) and Mouse Kidney (Lane 10)and NUMB major isoform 65 kDa was observed along with other isoforms between 53-70 kDa in all samples except Mouse Colon, Heart and Kidney. Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) b... View More
Product Details
PA5-21143
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A 17 amino acid peptide near the carboxy terminus of human NUMB.
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
0.02% sodium azide
Storage conditions
Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
RRID
AB_11154464
Product Specific Information
A suggested positive control is mouse lung tissue lysate.
PA5-21143 can be used with blocking peptide PEP-1257.
Target Information
Protein NUMB homolog is an evolutionarily conserved protein that controls the differentiation of neuronal progenitor cells. Recent studies have revealed that NUMB functions in clathrin-dependent endocytosis by binding to the AP-2 complex. NUMB is also expressed in postmitotic neurons and plays a role in axonal growth. Neural cells expressing Numb isoforms with short phosphotyrosine-binding (SPTB) domain undergo extensive neurite outgrowth, an effect that can be blocked by voltage-gated Ca2+ channel (VGCC) inhibitor or by Ca2+ chelator.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: h-Numb; m-Nb; m-numb; mnb; numb gene homolog; numb homolog; Protein numb homolog; Protein S171
Gene Aliases: c14_5527; C14orf41; Nb; NUMB; S171
UniProt ID: (Human) P49757, (Mouse) Q9QZS3
Entrez Gene ID: (Human) 8650, (Mouse) 18222, (Rat) 29419
Molecular Function:
scaffold/adaptor protein
axonogenesis
lateral ventricle development
neuroblast division in subventricular zone
positive regulation of cell migration
positive regulation of polarized epithelial cell differentiation
adherens junction organization
negative regulation of Notch signaling pathway
positive regulation of neurogenesis
lung epithelial cell differentiation
cell-cell adhesion
negative regulation of protein localization to plasma membrane
multicellular organism development
nervous system development
neuroblast proliferation
forebrain development
regulation of neuron differentiation
positive regulation of dendrite morphogenesis
cell proliferation
cell differentiation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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