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      NUMB Polyclonal Antibody

      See 7 other NUMB antibodies ›
      NUMB Polyclonal Antibody for Western Blot, IF, ICC and IHC

      Catalog#: PA5-21143 Invitrogen

      Catalog # Size Price (USD) Qty Datasheet
      PA5-21143 100 ug
      349.00
      Please enter a quantity in the range of 1 to 199.
      Suggested Secondary Antibodies

      Anti-Rabbit Secondary Antibodies (264)
      Alexa Fluor® Anti-Rabbit Secondary Antibodies (69)
      HRP Anti-Rabbit Secondary Antibodies (30)

      Product Details

      Bioinformatics

      Documents
      Details
      Tested species reactivity Human, Mouse, Rat
      Host / Isotype Rabbit / IgG
      Class Polyclonal
      Type Antibody
      Immunogen A 17 amino acid peptide near the carboxy terminus of human NUMB.
      Conjugate Unconjugated
      Form Liquid
      Concentration 1 mg/ml
      Purification Antigen affinity chromatography
      Storage buffer PBS
      Contains 0.02% sodium azide
      Storage Conditions Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
      Tested Applications Dilution *
      Immunocytochemistry (ICC) 20 ug/ml
      Immunofluorescence (IF) 20 µg/ml
      Immunohistochemistry (IHC) 5 µg/ml
      Western Blot (WB) 0.25-0.5 ug/ml

      * Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.

      Product Specific Information

      A suggested positive control is mouse lung tissue lysate.

      PA5-21143 can be used with blocking peptide PEP-1257.

      Background/Target Information

      NUMB, the mammalian homolog to the Drosophila asymmetric cell fate determinant NUMB, is thought to share several features molecular mechanisms in mammalian cells, generating asymmetric cell divisions during neurogenesis in vertebrate development as well as in hematopoietic stem cells. NUMB has been shown to inhibit Notch signaling, and is itself regulated by ubiquitinylation by MDM2. NUMB has also been shown to help activate the tumor suppressor p53, suggesting that loss of NUMB in cancerous cells would not only activate the potential oncogene Notch, but diminish the tumor suppressing effect of p53.

      For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

      Documents

      Material safety data sheets (MSDS)

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