|Immunocytochemistry (ICC)||20 ug/ml|
|Immunofluorescence (IF)||20 µg/ml|
|Immunohistochemistry (IHC)||5 µg/ml|
|Western Blot (WB)||0.25-0.5 ug/ml|
|Tested Species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A 17 amino acid peptide near the carboxy terminus of human NUMB.|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage conditions||Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C|
A suggested positive control is mouse lung tissue lysate.
PA5-21143 can be used with blocking peptide PEP-1257.
NUMB, the mammalian homolog to the Drosophila asymmetric cell fate determinant NUMB, is thought to share several features molecular mechanisms in mammalian cells, generating asymmetric cell divisions during neurogenesis in vertebrate development as well as in hematopoietic stem cells. NUMB has been shown to inhibit Notch signaling, and is itself regulated by ubiquitinylation by MDM2. NUMB has also been shown to help activate the tumor suppressor p53, suggesting that loss of NUMB in cancerous cells would not only activate the potential oncogene Notch, but diminish the tumor suppressing effect of p53.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: C14orf41, S171; h-Numb; m-Nb; numb gene homolog; numb homolog; Protein numb homolog; Protein S171
Gene Aliases: c14_5527; C14orf41; Nb; NUMB; S171
Molecular Function: signaling molecule
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