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Product Details
12329-1-AP
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Not Applicable
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
NUP98-NUP96 Fusion Protein Ag3016 (1515-1817 aa encoded by BC012906)
Conjugate
Form
Liquid
Concentration
0.27 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice (domestic); Dry ice (international)
Product Specific Information
This antibody was raised against the carboxy-terminal region of Nup98-Nup96, it recognizes all Nup98-Nup96 proteins except Nup98-N. Since the autoproteolysis is very rapid this antibody usually detected 98-115 kDa Nup96 protein in multiple cell lines. It is notable that RNA-editing deaminase 1 (Gene ID: 104), also named ADAR2, is a different protein from Nup98-Nup96 (ADAR2).
Immunogen sequence: TADPLDYRL SWHLWEVLRA LNYTHLSAQC EGVLQASYAG QLESEGLWEW AIFVLLHIDN SGIREKAVRE LLTRHCQLLE TPESWAKETF LTQKLRVPAK WIHEAKAVRA HMESDKHLEA LCLFKAEHWN RCHKLIIRHL ASDAIINENY DYLKGFLEDL APPERSSLIQ DWETSGLVYL DYIRVIEMLR HIQQVDCSGN DLEQLHIKVT SLCSRIEQIQ CYSAKDRLAQ SDMAKRVANL LRVVLSLHHP PDRTSDSTPD PQRVPLRLLA PHIGRLPMPE DYAMDELRSL TQSYLRELAV GSL (1515-1817 aa encoded by BC012906)
Target Information
Signal-mediated nuclear import and export proceed through the nuclear pore complex (NPC), which is comprised of approximately 50 unique proteins collectively known as nucleoporins. The 98 kDa nucleoporin is generated through a biogenesis pathway that involves synthesis and proteolytic cleavage of a 186 kDa precursor protein. This cleavage results in the 98 kDa nucleoporin as well as a 96 kDa nucleoporin, both of which are localized to the nucleoplasmic side of the NPC. Rat studies show that the 98 kDa nucleoporin functions as one of several docking site nucleoporins of transport substrates. The human gene has been shown to fuse to several genes following chromosome translocations in acute myelogenous leukemia (AML) and T-cell acute lymphocytic leukemia (T-ALL). This gene is one of several genes located in the imprinted gene domain of 11p15. 5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian, and breast cancer. Alternative splicing of this gene results in several transcript variants; however, not all variants have been fully described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: GLFG-repeat containing nucleoporin; Nuclear pore complex protein Nup98-Nup96; nucleoporin 98kD; nucleoporin 98kDa; Nup98-Nup96; NUP98/PHF23 fusion 2 protein; OTTHUMP00000013954
Gene Aliases: ADAR2; ADIR2; NUP196; NUP96; NUP98
UniProt ID: (Human) P52948
Entrez Gene ID: (Human) 4928
protein import into nucleus, docking
posttranscriptional tethering of RNA polymerase II gene DNA at nuclear periphery
DNA replication
RNA export from nucleus
mRNA export from nucleus
tRNA export from nucleus
nucleocytoplasmic transport
nuclear pore organization
sister chromatid cohesion
mitotic nuclear envelope disassembly
regulation of glucose transport
viral process
protein sumoylation
viral transcription
gene silencing by RNA
telomere tethering at nuclear periphery
nuclear pore complex assembly
intracellular transport of virus
regulation of cellular response to heat
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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