Invitrogen

Necdin Monoclonal Antibody (OTI5D1)

View all (14) Necdin antibodies

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Datasheet

Protocols

Questions & Answers

Tech Support

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Necdin Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of NDN in HeLa cells. Cells were probed with a NDN monoclonal antibody (Product # MA5-26294).

Necdin Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry was performed on paraffin-embedded adenocarcinoma of human endometrium tissue. To expose target proteins, 10mM citric buffer, pH6.0, 120°C for 3min was used. Following antigen retrieval, tissues were probed with a NDN monoclonal antibody (Product # MA5-26294).

Necdin Antibody in Western Blot (WB) — Western blot analysis of NDN in HEK293T cells in untransfected (Left lane) and transfected (Right lane) samples using 5 µg per lane. The samples were separated by SDS-PAGE and probed with NDN (Product # MA5-26294) monoclonal antibody.

Product Details

MA5-26294

Applications	Tested Dilution	Publications
Western Blot (WB)	1:4,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:150	-
Immunocytochemistry (ICC/IF)	1:100	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Mouse / IgG2a
Class	Monoclonal
Type	Antibody
Clone	OTI5D1
Immunogen	Full length human recombinant protein of NDN produced in HEK293T cell View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Affinity Chromatography
Storage buffer	PBS, pH 7.3, with 1% BSA, 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2724818

Target Information

This intronless gene is located in the Prader-Willi syndrome deletion region. It is an imprinted gene and is expressed exclusively from the paternal allele.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Necdin; necdin homolog; necdin, melanoma antigen (MAGE) family member; necdin-like protein; Prader-Willi syndrome chromosome region

Gene Aliases: HsT16328; NDN; PWCR

UniProt ID: (Human) Q99608

Entrez Gene ID: (Human) 4692

Molecular Function: scaffold/adaptor protein

Function(s)

neuron migration respiratory system process transcription from RNA polymerase II promoter nervous system development axonal fasciculation central nervous system development negative regulation of cell proliferation glial cell migration post-embryonic development sensory perception of pain regulation of growth positive regulation of transcription from RNA polymerase II promoter neurotrophin TRK receptor signaling pathway axon extension multicellular organismal homeostasis genetic imprinting

Process(es)

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding gamma-tubulin binding

Necdin Monoclonal Antibody (OTI5D1)

Product Details

MA5-26294

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Target Information

References (0)

Bioinformatics

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