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Invitrogen
NeuroD2 Polyclonal Antibody
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FIGURE: 1 / 4
NeuroD2 Antibody (PA5-104530) in ICC/IF
Immunocytochemistry analysis of NeuroD2 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with NeuroD2 polyclonal antibody (Product # PA5-104530) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-104530
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human NEUROD2(Accession Q15784), corresponding to amino acid residues K74-Q124.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total NDF2.
Target Information
Members of the myogenic determination family are basic helix-loop-helix (bHLH) proteins that can be separated into two classes, both of which work together to activate DNA transcription. Class A proteins include the ubiquitously expressed E-box binding factors, namely E2A, ITF-2 and HEB, while class B proteins, such as MyoD, myogenin and Neuro D (BETA2), are transiently expressed and exhibit a much more limited tissue distribution. Neuro D2 (neurogenic differentiation 2), also known as NDRF, NEUROD2 or bHLHa1, is a 382 amino acid nuclear protein that contains one bHLH domain and functions to induce neurogenic differentiation, playing an important role in the maintenance and determination of cell fate.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: bHLHa1; Brain bHLH protein KW8; Class A basic helix-loop-helix protein 1; NDF2; NDR2; NDRF; NeuroD-related factor; NeuroD2; neurogenic basic-helix-loop-helix protein; neurogenic differentiation 2; Neurogenic differentiation factor 2
Gene Aliases: BHLHA1; DEE72; EIEE72; NDRF; NEUROD2
UniProt ID: (Human) Q15784, (Mouse) Q62414, (Rat) Q63689
Entrez Gene ID: (Human) 4761, (Mouse) 18013, (Rat) 54276
RNA polymerase II regulatory region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein heterodimerization activity
protein dimerization activity
E-box binding
sequence-specific double-stranded DNA binding
transcription corepressor activity
protein binding
basic helix-loop-helix transcription factor
behavioral fear response
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
nervous system development
sensory organ development
associative learning
protein ubiquitination
cerebellar cortex development
cell differentiation
positive regulation of synaptic plasticity
positive regulation of neuron differentiation
positive regulation of transcription from RNA polymerase II promoter
neuron development
positive regulation of calcium-mediated signaling
positive regulation of sequence-specific DNA binding transcription factor activity
axon development
cellular response to electrical stimulus
cellular response to calcium ion
positive regulation of synapse maturation
negative regulation of synapse maturation
transcription, DNA-templated
transcription from RNA polymerase II promoter
multicellular organism development
negative regulation of nucleic acid-templated transcription
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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