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Immunofluorescent analysis of the neurofilament medium chain in paraffin-embedded human brain tissue (right) compared to a negative control without primary antibody (left). Tissue sections were deparaffinized with xylene, and rehydrated with ethanol. To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0) and microwaved for 8-15 min. Following antigen retrieval, tissues were washed with water and PBS, and then blocked in 0.3% BSA for 30 min at room temperature. Tissues were then probed with a neurofilament medium chain monoclonal antibody (Product # 13-0700) in 0.3% BSA at a dilution of 1:20 for 1 hour at 37°C. Tissues were then incubated with a Goat anti-Mouse IgG (H+L) Secondary Antibody, DyLight 488 conjugate for 1 hour at 37°C (green). Nuclei (blue) were stained with DAPI. Images were taken at 40X magnification.
|Tested species reactivity||Mouse , Rat , Human|
|Published species reactivity||Rat , Zebra fish , Chicken , Clawed frog|
|Host / Isotype||Mouse / IgG2a, kappa|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (IHC)||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Involved in the maintenance of neuronal caliber, neurofilaments are the intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. NF-M is the medium molecular weight microfilament subunit and runs on SDS-PAGE gels at approximately 160 kDa.
Neurofilament are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-H is the heavy or high molecular weight microfilament subunit and runs on SDS-PAGE gels in the range 180-220kDa, with some variation in different species. NF-H polyclonal antibody can be used to identify neurons and their processes in tissue sections and in tissue culture and the antibody can also be used to study microfilament accumulations seen in many neurological diseases, such as Lou Geri's disease or Alzheimer's disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Comprehensive evaluation of peripheral nerve regeneration in the acute healing phase using tissue clearing and optical microscopy in a rodent model.
13-0700 was used in immunocytochemistry to evaluate peripheral nerve regeneration during the acute wound healing phase
|Jung Y,Ng JH,Keating CP,Senthil-Kumar P,Zhao J,Randolph MA,Winograd JM,Evans CL||PloS one (9:null)||2014|
Metalloproteases and guidance of retinal axons in the developing visual system.
||Webber CA,Hocking JC,Yong VW,Stange CL,McFarlane S||The Journal of neuroscience : the official journal of the Society for Neuroscience (22:8091)||2002|
Exogenous growth factors induce the production of ganglion cells at the retinal margin.
||Fischer AJ,Dierks BD,Reh TA||Development (Cambridge, England) (129:2283)||2002|
Motoneuronal Sema3C is essential for setting stereotyped motor tract positioning in limb-derived chemotropic semaphorins.
||Sanyas I,Bozon M,Moret F,Castellani V||Development (Cambridge, England) (139:3633)||2012|
Ectopic Mitf in the embryonic chick retina by co-transfection of β-catenin and Otx2.
||Westenskow PD,McKean JB,Kubo F,Nakagawa S,Fuhrmann S||Investigative ophthalmology & visual science (51:5328)||2010|
Receptor tyrosine phosphatases guide vertebrate motor axons during development.
||Stepanek L,Stoker AW,Stoeckli E,Bixby JL||The Journal of neuroscience : the official journal of the Society for Neuroscience (25:3813)||2005|
Fgf signalling is required for formation of cartilage in the head.
13-0700 was used in immunohistochemistry to show that Fgf activity is required for the development of the oro-pharyngeal skeleton.
|Walshe J,Mason I||Developmental biology (264:522)||2003|
Wnt2b controls retinal cell differentiation at the ciliary marginal zone.
||Kubo F,Takeichi M,Nakagawa S||Development (Cambridge, England) (130:587)||2003|
Cues from neuroepithelium and surface ectoderm maintain neural crest-free regions within cranial mesenchyme of the developing chick.
||Golding JP,Dixon M,Gassmann M||Development (Cambridge, England) (129:1095)||2002|
Distinct regulatory cascades for head and trunk myogenesis.
||Mootoosamy RC,Dietrich S||Development (Cambridge, England) (129:573)||2002|
A critical role for sonic hedgehog signaling in the early expansion of the developing brain.
||Britto J,Tannahill D,Keynes R||Nature neuroscience (5:103)||2002|
Boundary formation and compartition in the avian diencephalon.
||Larsen CW,Zeltser LM,Lumsden A||The Journal of neuroscience : the official journal of the Society for Neuroscience (21:4699)||2001|
Multiple actions of neurturin correlate with spatiotemporal patterns of Ret expression in developing chick cranial ganglion neurons.
||Hashino E,Johnson EM,Milbrandt J,Shero M,Salvi RJ,Cohan CS||The Journal of neuroscience : the official journal of the Society for Neuroscience (19:8476)||1999|
NF160, NFM, NEF3, NF-M, Nef3, NF165, Nfm
160 kDa neurofilament protein, neurofilament 3, medium, neurofilament medium polypeptide, neurofilament protein M, neurofilament triplet M protein, neurofilament-M, NF-M, Neurofilament protein, middle polypeptide, neurofilament 3, neurofilament, medium polypeptide 150kDa, neurofilament-3 (150 kD medium), NEF3, NFM, neurofilament, medium polypeptide 150kDa