This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
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Immunogen sequence: SPESVDLVNE ELKGKVLGLS RDPAKVAEED EDDDGGIMMR SKETSSPGTD DVFTPAPSDS PSSQRIQRCL SDPGPHPEPG EGEPFFPKG
Highest antigen sequence identity to the following orthologs: Mouse - 83%, Rat - 83%.
HIER pH 6 was used for IHC antigen retrieval. Cells used for ICC/IF were permeabilized and fixed with PFA/Triton™ X-100.
The Na+/H+ antiporter (Nhe-1) is a ubiquitous membrane-bound enzyme involved in pH regulation of vertebrate cells and is specifically inhibited by the diuretic drug amiloride and activated by a variety of signals including growth factors, mitogens, neurotransmitters, and tumor promoters. Nhe-1 acts as an anchor for actin filaments to control the integrity of the cortical cytoskeleton. This occurs through a previously unrecognized structural link between Nhe-1 and the actin-binding proteins ezrin, radixin, and moesin, collectively referred to as ERM proteins. A structural role for Nhe-1 has been proposed in regulating the cortical cytoskeleton that is independent of its function as an ion exchanger. It is also thought that Nhe-1 play a role in hypertension. At least two isoforms of Nhe-1 are known to exist.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: APNH; Na(+)/H(+) antiporter, amiloride-sensitive; Na(+)/H(+) exchanger 1; Na-Li countertransporter; NHE-1; protein phosphatase 1, regulatory subunit 143; Sodium/hydrogen exchanger 1; solute carrier family 9 (sodium/hydrogen exchanger), isoform 1 (antiporter, Na+/H+, amiloride sensitive); solute carrier family 9 (sodium/hydrogen exchanger), member 1 (antiporter, Na+/H+, amiloride sensitive); Solute carrier family 9 member 1; solute carrier family 9, subfamily A (NHE1, cation proton antiporter 1), member 1
Gene Aliases: APNH; APNH1; LIKNS; NHE-1; NHE1; PPP1R143; SLC9A1
UniProt ID: (Human) P19634
Entrez Gene ID: (Human) 6548