Invitrogen

Nicastrin Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

8 Published Figures

11 References

View all (18) Nicastrin antibodies

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Datasheet

Protocols

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FIGURE: 1 / 14

Nicastrin Antibody (PA1-758) in WB

Western blot was performed using Anti-Nicastrin Polyclonal Antibody (Product # PA1-758) and a 120 kDa band corresponding to Nicastrin was observed across cell lines and tissues tested along with an uncharacterized band (*) at 55 kDa. Whole cell extracts (60 µg lysate) of HeLa (Lane 1), IMR-32 (Lane 2), NIH/3T3 (Lane 3), L6 (Lane 4), Mouse Brain (Lane 5) and Rat Brain (Lane 6) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product #... View More

Product Image Gallery

Nicastrin Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with Nicastrin siRNA and decrease in signal intensity was observed in Western Blot application using Anti-Nicastrin Polyclonal Antibody (Product # PA1-758). {KD}

Product Details

PA1-758

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

View 11 publications 11 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic Peptide: C(688) I N A K A D V L F I A P R E P G A V S Y(708)

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2149570

Product Specific Information

PA1-758 detects nicastrin from human, mouse and rat samples.

PA1-758 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~110 kDa protein representing nicastrin.

PA1-758 immunizing peptide corresponds to amino acid residues 688-708 from human nicastrin. There is a single amino acid substitution between the human and mouse sequence. PA1-758 immunizing peptide (Cat. # PEP-131) is available for use in neutralization and control experiments.

Target Information

This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Upregulation of APP endocytosis by neuronal aging drives amyloid-dependent synapse loss.

Journal of cell science

Burrinha T,Martinsson I,Gomes R,Terrasso AP,Gouras GK,Almeida CG

PA1-758 was used in Western Blot to identify APP endocytosis upregulation as a potential mechanism of neuronal aging and, thus, a novel target to prevent late-onset Alzheimer's disease.

Sat May 01 00:00:00 EDT 2021

Bin1 and CD2AP polarise the endocytic generation of beta-amyloid.

EMBO reports

Ubelmann F,Burrinha T,Salavessa L,Gomes R,Ferreira C,Moreno N,Guimas Almeida C

Published figure using Nicastrin polyclonal antibody (Product # PA1-758) in Western Blot

Sun Jan 01 00:00:00 EST 2017

Presenilin transmembrane domain 8 conserved AXXXAXXXG motifs are required for the activity of the γ-secretase complex.

The Journal of biological chemistry

Marinangeli C,Tasiaux B,Opsomer R,Hage S,Sodero AO,Dewachter I,Octave JN,Smith SO,Constantinescu SN,Kienlen-Campard P

Published figure using Nicastrin polyclonal antibody (Product # PA1-758) in Western Blot

Fri Mar 13 00:00:00 EDT 2015

Ubiquilin-1 modulates γ-secretase-mediated ε-site cleavage in neuronal cells.

Biochemistry

Viswanathan J,Haapasalo A,Kurkinen KM,Natunen T,Mäkinen P,Bertram L,Soininen H,Tanzi RE,Hiltunen M

PA1-758 was used in western blot to study the ability of a variant of ubiquilin-1 to increase gamma-secretase-mediated cleavage of APP at the epsilon site

Tue Jun 04 00:00:00 EDT 2013

Chronic administration of anti-stroke herbal medicine TongLuoJiuNao reduces amyloidogenic processing of amyloid precursor protein in a mouse model of Alzheimer's disease.

PloS one

He P,Li P,Hua Q,Liu Y,Staufenbiel M,Li R,Shen Y

Published figure using Nicastrin polyclonal antibody (Product # PA1-758) in Western Blot

Tue Dec 03 00:00:00 EST 2013

Presenilins regulate neurotrypsin gene expression and neurotrypsin-dependent agrin cleavage via cyclic AMP response element-binding protein (CREB) modulation.

The Journal of biological chemistry

Almenar-Queralt A,Kim SN,Benner C,Herrera CM,Kang DE,Garcia-Bassets I,Goldstein LS

PA1-758 was used in western blot to study the role of CREB in the mechanism by which neurotrypsin expression and agarin proteolysis is modulated by presenilins

Fri Dec 06 00:00:00 EST 2013

Presenilins regulate neurotrypsin gene expression and neurotrypsin-dependent agrin cleavage via cyclic AMP response element-binding protein (CREB) modulation.

The Journal of biological chemistry

Almenar-Queralt A,Kim SN,Benner C,Herrera CM,Kang DE,Garcia-Bassets I,Goldstein LS

PA1-758 was used in western blot to study the role of CREB in the mechanism by which neurotrypsin expression and agarin proteolysis is modulated by presenilins

Fri Dec 06 00:00:00 EST 2013

Potent amyloidogenicity and pathogenicity of Aβ43.

Nature neuroscience

Saito T,Suemoto T,Brouwers N,Sleegers K,Funamoto S,Mihira N,Matsuba Y,Yamada K,Nilsson P,Takano J,Nishimura M,Iwata N,Van Broeckhoven C,Ihara Y,Saido TC

PA1-758 was used in western blot to investigate the contribution of A beta 43 to Alzheimer disease pathology

Sun Jul 03 00:00:00 EDT 2011

Calsenilin regulates presenilin 1/γ-secretase-mediated N-cadherin ε-cleavage and β-catenin signaling.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Jang C,Choi JK,Na YJ,Jang B,Wasco W,Buxbaum JD,Kim YS,Choi EK

PA1-758 was used in western blot to identify the role of Calsenilin in N-cadherin cleavage in neuroblastomas

Thu Dec 01 00:00:00 EST 2011

Ubiquilin 1 modulates amyloid precursor protein trafficking and Abeta secretion.

The Journal of biological chemistry

Hiltunen M,Lu A,Thomas AV,Romano DM,Kim M,Jones PB,Xie Z,Kounnas MZ,Wagner SL,Berezovska O,Hyman BT,Tesco G,Bertram L,Tanzi RE

PA1-758 was used in western blot to investigate the role of ubiquilin 1 during the amyloid precursor protein trafficking and Abeta secretion.

Fri Oct 27 00:00:00 EDT 2006

The presenilin proteins are components of multiple membrane-bound complexes that have different biological activities.

The Journal of biological chemistry

Gu Y,Sanjo N,Chen F,Hasegawa H,Petit A,Ruan X,Li W,Shier C,Kawarai T,Schmitt-Ulms G,Westaway D,St George-Hyslop P,Fraser PE

PA1-758 was used in western blot to study multiple membrane-bound presenilin complexes and their biological activities.

Fri Jul 23 00:00:00 EDT 2004

APH-1 interacts with mature and immature forms of presenilins and nicastrin and may play a role in maturation of presenilin.nicastrin complexes.

The Journal of biological chemistry

Gu Y,Chen F,Sanjo N,Kawarai T,Hasegawa H,Duthie M,Li W,Ruan X,Luthra A,Mount HT,Tandon A,Fraser PE,St George-Hyslop P

Published figure using Nicastrin polyclonal antibody (Product # PA1-758) in Western Blot

Fri Feb 28 00:00:00 EST 2003

APH-1 interacts with mature and immature forms of presenilins and nicastrin and may play a role in maturation of presenilin.nicastrin complexes.

The Journal of biological chemistry

Gu Y,Chen F,Sanjo N,Kawarai T,Hasegawa H,Duthie M,Li W,Ruan X,Luthra A,Mount HT,Tandon A,Fraser PE,St George-Hyslop P

Published figure using Nicastrin polyclonal antibody (Product # PA1-758) in Western Blot

Fri Feb 28 00:00:00 EST 2003

Bioinformatics

Protein Aliases: anterior pharynx-defective 2; Nicastrin; OTTHUMP00000031840

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Gene Aliases: 9430068N19Rik; AA727311; Aph2; ATAG1874; D1Dau13e; KIAA0253; mKIAA0253; NCSTN; Nct; UNQ1874/PRO4317

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UniProt ID: (Human) Q92542, (Mouse) P57716, (Rat) Q8CGU6

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Entrez Gene ID: (Human) 23385, (Mouse) 59287, (Rat) 289231

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Function(s)

myeloid cell homeostasis proteolysis membrane protein ectodomain proteolysis Notch signaling pathway protein processing T cell proliferation positive regulation of catalytic activity beta-amyloid metabolic process epithelial cell proliferation Notch receptor processing membrane protein intracellular domain proteolysis amyloid precursor protein catabolic process positive regulation of apoptotic process ephrin receptor signaling pathway

Process(es)

endopeptidase activity protein binding peptidase activity

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Nicastrin Polyclonal Antibody

Nicastrin Antibody (PA1-758) in WB

Product Details

PA1-758

Western Blot (WB)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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