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Invitrogen
Nicastrin Polyclonal Antibody
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FIGURE: 1 / 14
Nicastrin Antibody (PA1-758) in WB
Western blot was performed using Anti-Nicastrin Polyclonal Antibody (Product # PA1-758) and a 120 kDa band corresponding to Nicastrin was observed across cell lines and tissues tested along with an uncharacterized band (*) at 55 kDa. Whole cell extracts (60 µg lysate) of HeLa (Lane 1), IMR-32 (Lane 2), NIH/3T3 (Lane 3), L6 (Lane 4), Mouse Brain (Lane 5) and Rat Brain (Lane 6) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product #... View More
Product Details
PA1-758
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic Peptide: C(688) I N A K A D V L F I A P R E P G A V S Y(708)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA1-758 detects nicastrin from human, mouse and rat samples.
PA1-758 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~110 kDa protein representing nicastrin.
PA1-758 immunizing peptide corresponds to amino acid residues 688-708 from human nicastrin. There is a single amino acid substitution between the human and mouse sequence. PA1-758 immunizing peptide (Cat. # PEP-131) is available for use in neutralization and control experiments.
Target Information
NCSTN (Nicastrin) is a gene that encodes a type I transmembrane glycoprotein that is an integral component of the multimeric gamma-secretase complex. The encoded protein cleaves integral membrane proteins, including Notch receptors and beta-amyloid precursor protein, and may be a stabilizing cofactor required for gamma-secretase complex assembly. The cleavage of beta-amyloid precursor protein yields amyloid beta peptide, the main component of the neuritic plaque and the hallmark lesion in the brains of patients with Alzheimer's disease; however, the nature of the encoded protein's role in Alzheimer's disease is not known for certain. Mutations in this gene are associated with familial acne inversa. A pseudogene of this gene is present on chromosome 21.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: anterior pharynx-defective 2; KIAA0253; Nicastrin; OTTHUMP00000031840; type 1 transmembrane protein; unnamed protein product
Gene Aliases: 9430068N19Rik; AA727311; Aph2; ATAG1874; D1Dau13e; KIAA0253; mKIAA0253; NCSTN; Nct; UNQ1874/PRO4317
UniProt ID: (Human) Q92542, (Mouse) P57716, (Rat) Q8CGU6
Entrez Gene ID: (Human) 23385, (Mouse) 59287, (Rat) 289231
myeloid cell homeostasis
proteolysis
membrane protein ectodomain proteolysis
Notch signaling pathway
protein processing
T cell proliferation
positive regulation of catalytic activity
beta-amyloid metabolic process
epithelial cell proliferation
dopamine receptor signaling pathway
glutamate receptor signaling pathway
Notch receptor processing
learning or memory
cerebellum development
central nervous system myelination
adult behavior
membrane protein intracellular domain proteolysis
beta-amyloid formation
amyloid precursor protein metabolic process
amyloid precursor protein biosynthetic process
positive regulation of amyloid precursor protein biosynthetic process
amyloid precursor protein catabolic process
neuron apoptotic process
cellular response to calcium ion
regulation of long-term synaptic potentiation
short-term synaptic potentiation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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