Invitrogen

Nicastrin Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated. View Details

5 Published Figures

9 References

View all (16) Nicastrin antibodies

Datasheet

Questions & Answers

Datasheet

Questions & Answers

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Nicastrin Antibody in Western Blot (WB) — Western blot analysis was performed on membrane extracts (30 µglysate) of T98G (Lane 1), and RSC96 (Lane 2). The blots were probed with Rabbit Anti-Nicastrin Polyclonal Antibody (Product # PA1-758, 2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 120 kDa band corresponding to immature Nicastrin was observed across the cell lines tested. In addition, a 140 kDa band corresponding to mature, glycosylated Nicastrin was observed in RSC96. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

Nicastrin Antibody in Knockdown — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with Nicastrin siRNA and decrease in signal intensity was observed in Western Blot application using Anti-Nicastrin Polyclonal Antibody (Product # PA1-758). {KD}

Nicastrin Antibody — Published figure using Nicastrin polyclonal antibody (Product # PA1-758)

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

View 9 publications 9 publications

Product Specifications

Species

Human, Mouse, Rat

Published species

Human, Mouse

Expression System

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic Peptide: C(688) I N A K A D V L F I A P R E P G A V S Y(708)

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2149570

Product Specific Information

PA1-758 detects nicastrin from human, mouse and rat samples.

PA1-758 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~110 kDa protein representing nicastrin.

PA1-758 immunizing peptide corresponds to amino acid residues 688-708 from human nicastrin. There is a single amino acid substitution between the human and mouse sequence. PA1-758 immunizing peptide (Cat. # PEP-131) is available for use in neutralization and control experiments.

Target Information

This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.