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      Nicastrin Polyclonal Antibody

      See 3 other Nicastrin antibodies ›
      Nicastrin Polyclonal Antibody for Western Blot

      Catalog#: PA1-758 Invitrogen

      Catalog # Size Price (USD) Qty Datasheet
      PA1-758 100 µg
      309.00
      Please enter a quantity in the range of 1 to 199.
      Suggested Secondary Antibodies

      Anti-Rabbit Secondary Antibodies (264)
      Alexa Fluor® Anti-Rabbit Secondary Antibodies (69)
      HRP Anti-Rabbit Secondary Antibodies (30)

      Product Details

      References

      Bioinformatics

      Documents
      Details
      Tested species reactivity Human, Mouse, Rat
      Published species reactivity Human, Mouse
      Host / Isotype Rabbit / IgG
      Class Polyclonal
      Type Antibody
      Immunogen Synthetic Peptide: C(688) I N A K A D V L F I A P R E P G A V S Y(708)
      Conjugate Unconjugated
      Form Liquid
      Concentration 1 mg/ml
      Purification Antigen affinity chromatography
      Storage buffer PBS with 1mg/ml BSA
      Contains 0.05% sodium azide
      Storage Conditions -20° C, Avoid Freeze/Thaw Cycles
      Tested Applications Dilution *
      Western Blot (WB) 2 µg/ml

      * Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.

      Published Applications
      Western Blot (WB) See 9 publications below

      Product Specific Information

      PA1-758 detects nicastrin from human, mouse and rat samples.

      PA1-758 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~110 kDa protein representing nicastrin.

      PA1-758 immunizing peptide corresponds to amino acid residues 688-708 from human nicastrin. There is a single amino acid substitution between the human and mouse sequence. PA1-758 immunizing peptide (Cat. # PEP-131) is available for use in neutralization and control experiments.

      Background/Target Information

      Nicastrin, in addition to presenilin, PEN2, and APH-1 forms the -gamma-secretase protein complex, a membrane-bound aspartyl protease that can cleave certain proteins at peptide bonds buried within the hydrophobic environment of the lipid bilayer. This cleavage is responsible for a key step in signaling from several cell-surface receptors and is thought to be required for the generation of the neurotoxic amyloid peptides that are central to the pathogenesis of Alzheimer and quote;s disease. Like the tumor necrosis factor-alpha-converting enzyme (TACE) and the beta-site cleavage enzyme (BACE) protease families, -gamma-secretase will cleave the amyloid precursor protein (APP), but within the intramembrane region of APP, resulting in either the non-toxic p3 (from the alpha and -gamma cleavage site) or the toxic Abeta amyloid peptide (from the beta and -gamma cleavage site). It is thought that accumulation of the Abeta peptide is the precursor to Alzheimer and quote;s disease. Nicastrin is also thought to be involved in cell proliferation and signaling, especially in regards to activation of Notch receptors as loss of Nicastrin expression results in mouse embryonic lethality.

      For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

      References

      Western Blot

      Species Dilution Title Authors Journal (Vol:Pg) Year
      Human Not Cited Presenilin transmembrane domain 8 conserved AXXXAXXXG motifs are required for the activity of the ¿-secretase complex.
      PA1-758 was used in western blot to investigate presenilin assembly and catalytic activity       		Marinangeli C,Tasiaux B,Opsomer R,Hage S,Sodero AO,Dewachter I,Octave JN,Smith SO,Constantinescu SN,Kienlen-Campard P The Journal of biological chemistry (290:7169) 2015
      Mouse Not Cited Presenilins regulate neurotrypsin gene expression and neurotrypsin-dependent agrin cleavage via cyclic AMP response element-binding protein (CREB) modulation.
      PA1-758 was used in western blot to study the role of CREB in the mechanism by which neurotrypsin expression and agarin proteolysis is modulated by presenilins       		Almenar-Queralt A,Kim SN,Benner C,Herrera CM,Kang DE,Garcia-Bassets I,Goldstein LS The Journal of biological chemistry (288:35222) 2013
      Human 1:1000 Ubiquilin-1 modulates ¿-secretase-mediated ¿-site cleavage in neuronal cells.
      PA1-758 was used in western blot to study the ability of a variant of ubiquilin-1 to increase gamma-secretase-mediated cleavage of APP at the epsilon site       		Viswanathan J,Haapasalo A,Kurkinen KM,Natunen T,Mäkinen P,Bertram L,Soininen H,Tanzi RE,Hiltunen M Biochemistry (52:3899) 2013
      Mouse 1:1000 Chronic administration of anti-stroke herbal medicine TongLuoJiuNao reduces amyloidogenic processing of amyloid precursor protein in a mouse model of Alzheimer's disease.
      PA1-758 was used in western blot to study the effects of the herbal medicine TongLuoJuiNao on levels of BACE1, gamma secretase components, and beta-amyloid production in a murine model of Alzheimer's disease       		He P,Li P,Hua Q,Liu Y,Staufenbiel M,Li R,Shen Y PloS one (8:null) 2013
      Mouse Not Cited Calsenilin regulates presenilin 1/¿-secretase-mediated N-cadherin ¿-cleavage and ß-catenin signaling.
      PA1-758 was used in western blot to identify the role of Calsenilin in N-cadherin cleavage in neuroblastomas       		Jang C,Choi JK,Na YJ,Jang B,Wasco W,Buxbaum JD,Kim YS,Choi EK FASEB journal : official publication of the Federation of American Societies for Experimental Biology (25:4174) 2011
      Mouse Not Cited Potent amyloidogenicity and pathogenicity of Aß43.
      PA1-758 was used in western blot to investigate the contribution of A beta 43 to Alzheimer disease pathology       		Saito T,Suemoto T,Brouwers N,Sleegers K,Funamoto S,Mihira N,Matsuba Y,Yamada K,Nilsson P,Takano J,Nishimura M,Iwata N,Van Broeckhoven C,Ihara Y,Saido TC Nature neuroscience (14:1023) 2011
      Human Not Cited Ubiquilin 1 modulates amyloid precursor protein trafficking and Abeta secretion.
      PA1-758 was used in western blot to investigate the role of ubiquilin 1 during the amyloid precursor protein trafficking and Abeta secretion.       		Hiltunen M,Lu A,Thomas AV,Romano DM,Kim M,Jones PB,Xie Z,Kounnas MZ,Wagner SL,Berezovska O,Hyman BT,Tesco G,Bertram L,Tanzi RE The Journal of biological chemistry (281:32240) 2006
      Human Not Cited The presenilin proteins are components of multiple membrane-bound complexes that have different biological activities.
      PA1-758 was used in western blot to study multiple membrane-bound presenilin complexes and their biological activities.       		Gu Y,Sanjo N,Chen F,Hasegawa H,Petit A,Ruan X,Li W,Shier C,Kawarai T,Schmitt-Ulms G,Westaway D,St George-Hyslop P,Fraser PE The Journal of biological chemistry (279:31329) 2004
      Human Not Cited APH-1 interacts with mature and immature forms of presenilins and nicastrin and may play a role in maturation of presenilin.nicastrin complexes.
      PA1-758 was used in western blot to investigate the role of APH-1 during the formation of presenilin and nicastrin complexes.       		Gu Y,Chen F,Sanjo N,Kawarai T,Hasegawa H,Duthie M,Li W,Ruan X,Luthra A,Mount HT,Tandon A,Fraser PE,St George-Hyslop P The Journal of biological chemistry (278:7374) 2003

      Documents

      Material safety data sheets (MSDS)

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