Invitrogen

Nicastrin Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

8 Published Figures

10 References

View all (19) Nicastrin antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

Nicastrin Antibody in Western Blot (WB) — Western blot analysis was performed on membrane extracts (30 µglysate) of T98G (Lane 1), and RSC96 (Lane 2). The blots were probed with Rabbit Anti-Nicastrin Polyclonal Antibody (Product # PA1-758, 2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 120 kDa band corresponding to immature Nicastrin was observed across the cell lines tested. In addition, a 140 kDa band corresponding to mature, glycosylated Nicastrin was observed in RSC96. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

Nicastrin Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with Nicastrin siRNA and decrease in signal intensity was observed in Western Blot application using Anti-Nicastrin Polyclonal Antibody (Product # PA1-758). {KD}

Product Details

PA1-758

Applications	Tested Dilution	Publications
Western Blot (WB)	1-2 µg/mL	View 10 publications 10 publications

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthetic Peptide: C(688) I N A K A D V L F I A P R E P G A V S Y(708)
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS with 1mg/mL BSA
Contains	0.05% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2149570

Product Specific Information

PA1-758 detects nicastrin from human, mouse and rat samples.

PA1-758 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~110 kDa protein representing nicastrin.

PA1-758 immunizing peptide corresponds to amino acid residues 688-708 from human nicastrin. There is a single amino acid substitution between the human and mouse sequence. PA1-758 immunizing peptide (Cat. # PEP-131) is available for use in neutralization and control experiments.

Target Information

This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Bin1 and CD2AP polarise the endocytic generation of beta-amyloid.

EMBO reports

Ubelmann F,Burrinha T,Salavessa L,Gomes R,Ferreira C,Moreno N,Guimas Almeida C

Published figure using Nicastrin polyclonal antibody (Product # PA1-758) in Western Blot

Sun Jan 01 00:00:00 EST 2017

Presenilin transmembrane domain 8 conserved AXXXAXXXG motifs are required for the activity of the γ-secretase complex.

The Journal of biological chemistry

Marinangeli C,Tasiaux B,Opsomer R,Hage S,Sodero AO,Dewachter I,Octave JN,Smith SO,Constantinescu SN,Kienlen-Campard P

PA1-758 was used in western blot to investigate presenilin assembly and catalytic activity

Fri Mar 13 00:00:00 EDT 2015

Presenilins regulate neurotrypsin gene expression and neurotrypsin-dependent agrin cleavage via cyclic AMP response element-binding protein (CREB) modulation.

The Journal of biological chemistry

Almenar-Queralt A,Kim SN,Benner C,Herrera CM,Kang DE,Garcia-Bassets I,Goldstein LS

PA1-758 was used in western blot to study the role of CREB in the mechanism by which neurotrypsin expression and agarin proteolysis is modulated by presenilins

Fri Dec 06 00:00:00 EST 2013

Presenilins regulate neurotrypsin gene expression and neurotrypsin-dependent agrin cleavage via cyclic AMP response element-binding protein (CREB) modulation.

The Journal of biological chemistry

Almenar-Queralt A,Kim SN,Benner C,Herrera CM,Kang DE,Garcia-Bassets I,Goldstein LS

PA1-758 was used in western blot to study the role of CREB in the mechanism by which neurotrypsin expression and agarin proteolysis is modulated by presenilins

Fri Dec 06 00:00:00 EST 2013

Chronic administration of anti-stroke herbal medicine TongLuoJiuNao reduces amyloidogenic processing of amyloid precursor protein in a mouse model of Alzheimer's disease.

PloS one

He P,Li P,Hua Q,Liu Y,Staufenbiel M,Li R,Shen Y

PA1-758 was used in western blot to study the effects of the herbal medicine TongLuoJuiNao on levels of BACE1, gamma secretase components, and beta-amyloid production in a murine model of Alzheimer's disease

Tue Dec 03 00:00:00 EST 2013

Ubiquilin-1 modulates γ-secretase-mediated ε-site cleavage in neuronal cells.

Biochemistry

Viswanathan J,Haapasalo A,Kurkinen KM,Natunen T,Mäkinen P,Bertram L,Soininen H,Tanzi RE,Hiltunen M

PA1-758 was used in western blot to study the ability of a variant of ubiquilin-1 to increase gamma-secretase-mediated cleavage of APP at the epsilon site

Tue Jun 04 00:00:00 EDT 2013

Calsenilin regulates presenilin 1/γ-secretase-mediated N-cadherin ε-cleavage and β-catenin signaling.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Jang C,Choi JK,Na YJ,Jang B,Wasco W,Buxbaum JD,Kim YS,Choi EK

PA1-758 was used in western blot to identify the role of Calsenilin in N-cadherin cleavage in neuroblastomas

Thu Dec 01 00:00:00 EST 2011

Potent amyloidogenicity and pathogenicity of Aβ43.

Nature neuroscience

Saito T,Suemoto T,Brouwers N,Sleegers K,Funamoto S,Mihira N,Matsuba Y,Yamada K,Nilsson P,Takano J,Nishimura M,Iwata N,Van Broeckhoven C,Ihara Y,Saido TC

PA1-758 was used in western blot to investigate the contribution of A beta 43 to Alzheimer disease pathology

Sun Jul 03 00:00:00 EDT 2011

Ubiquilin 1 modulates amyloid precursor protein trafficking and Abeta secretion.

The Journal of biological chemistry

Hiltunen M,Lu A,Thomas AV,Romano DM,Kim M,Jones PB,Xie Z,Kounnas MZ,Wagner SL,Berezovska O,Hyman BT,Tesco G,Bertram L,Tanzi RE

PA1-758 was used in western blot to investigate the role of ubiquilin 1 during the amyloid precursor protein trafficking and Abeta secretion.

Fri Oct 27 00:00:00 EDT 2006

The presenilin proteins are components of multiple membrane-bound complexes that have different biological activities.

The Journal of biological chemistry

Gu Y,Sanjo N,Chen F,Hasegawa H,Petit A,Ruan X,Li W,Shier C,Kawarai T,Schmitt-Ulms G,Westaway D,St George-Hyslop P,Fraser PE

PA1-758 was used in western blot to study multiple membrane-bound presenilin complexes and their biological activities.

Fri Jul 23 00:00:00 EDT 2004

APH-1 interacts with mature and immature forms of presenilins and nicastrin and may play a role in maturation of presenilin.nicastrin complexes.

The Journal of biological chemistry

Gu Y,Chen F,Sanjo N,Kawarai T,Hasegawa H,Duthie M,Li W,Ruan X,Luthra A,Mount HT,Tandon A,Fraser PE,St George-Hyslop P

PA1-758 was used in western blot to investigate the role of APH-1 during the formation of presenilin and nicastrin complexes.

Fri Feb 28 00:00:00 EST 2003

APH-1 interacts with mature and immature forms of presenilins and nicastrin and may play a role in maturation of presenilin.nicastrin complexes.

The Journal of biological chemistry

Gu Y,Chen F,Sanjo N,Kawarai T,Hasegawa H,Duthie M,Li W,Ruan X,Luthra A,Mount HT,Tandon A,Fraser PE,St George-Hyslop P

PA1-758 was used in western blot to investigate the role of APH-1 during the formation of presenilin and nicastrin complexes.

Fri Feb 28 00:00:00 EST 2003

Bioinformatics

Protein Aliases: anterior pharynx-defective 2; Nicastrin; OTTHUMP00000031840

Gene Aliases: 9430068N19Rik; AA727311; Aph2; ATAG1874; D1Dau13e; KIAA0253; mKIAA0253; NCSTN; Nct; UNQ1874/PRO4317

UniProt ID: (Human) Q92542, (Mouse) P57716, (Rat) Q8CH12

Entrez Gene ID: (Human) 23385, (Mouse) 59287, (Rat) 289231

Function(s)

myeloid cell homeostasis proteolysis membrane protein ectodomain proteolysis Notch signaling pathway protein processing T cell proliferation positive regulation of catalytic activity beta-amyloid metabolic process epithelial cell proliferation Notch receptor processing membrane protein intracellular domain proteolysis amyloid precursor protein catabolic process positive regulation of apoptotic process ephrin receptor signaling pathway

Process(es)

endopeptidase activity protein binding peptidase activity

Nicastrin Polyclonal Antibody

Product Details

PA1-758

Western Blot (WB)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Brands

Shopping Tools

eSolutions

Support

Thermo Fisher Scientific

Your items have has been added!