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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • Nicastrin Antibodies

          Invitrogen

          Nicastrin Polyclonal Antibody

          Advanced Verification

          This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

          8 Published Figures
          11 References
          View all (18) Nicastrin antibodies
          Datasheet
          Protocols
          Questions & Answers
          Tech Support

          Cite Nicastrin Polyclonal Antibody

          Datasheet
          Protocols
          Questions & Answers
          Tech Support
          • Antibody Testing Data (4)
          • Published Figures (8)
          • Advanced Verification (2)
          Nicastrin Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 14

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          Nicastrin Antibody (PA1-758) in WB

          Western blot was performed using Anti-Nicastrin Polyclonal Antibody (Product # PA1-758) and a 120 kDa band corresponding to Nicastrin was observed across cell lines and tissues tested along with an uncharacterized band (*) at 55 kDa. Whole cell extracts (60 µg lysate) of HeLa (Lane 1), IMR-32 (Lane 2), NIH/3T3 (Lane 3), L6 (Lane 4), Mouse Brain (Lane 5) and Rat Brain (Lane 6) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product #... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          Product Details

          PA1-758

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1-2 µg/mL
          View 11 publications 11 publications
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Published species

          Human, Mouse

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Synthetic Peptide: C(688) I N A K A D V L F I A P R E P G A V S Y(708)
          View immunogen

          Conjugate

          Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS with 1mg/mL BSA

          Contains

          0.05% sodium azide

          Storage conditions

          -20° C, Avoid Freeze/Thaw Cycles

          RRID

          AB_2149570

          Product Specific Information

          PA1-758 detects nicastrin from human, mouse and rat samples.

          PA1-758 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~110 kDa protein representing nicastrin.

          PA1-758 immunizing peptide corresponds to amino acid residues 688-708 from human nicastrin. There is a single amino acid substitution between the human and mouse sequence. PA1-758 immunizing peptide (Cat. # PEP-131) is available for use in neutralization and control experiments.

          Target Information

          This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: anterior pharynx-defective 2; Nicastrin; OTTHUMP00000031840

          View more View less

          Gene Aliases: 9430068N19Rik; AA727311; Aph2; ATAG1874; D1Dau13e; KIAA0253; mKIAA0253; NCSTN; Nct; UNQ1874/PRO4317

          View more View less

          UniProt ID: (Human) Q92542, (Mouse) P57716, (Rat) Q8CGU6

          View more View less

          Entrez Gene ID: (Human) 23385, (Mouse) 59287, (Rat) 289231

          View more View less

          Function(s)
          myeloid cell homeostasis proteolysis membrane protein ectodomain proteolysis Notch signaling pathway protein processing T cell proliferation positive regulation of catalytic activity beta-amyloid metabolic process epithelial cell proliferation Notch receptor processing membrane protein intracellular domain proteolysis amyloid precursor protein catabolic process positive regulation of apoptotic process ephrin receptor signaling pathway
          Process(es)
          endopeptidase activity protein binding peptidase activity
          It has to be done as per old AB suggested Products section.
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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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