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Nidogen Antibody (PA5-97016) in WB
Western blot analysis of Nidogen in extracts of various cell lines. Samples were incubated with Nidogen Polyclonal antibody (Product # PA5-97016) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
Product Details
PA5-97016
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 110-400 of human NID1 (NP_0024992)
Conjugate
Form
Liquid
Concentration
0.66 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2808818
Product Specific Information
Immunogen sequence: DLDTTDGLGK VYYREDLSPS ITQRAAECVH RGFPEISFQP SSAVVVTWES VAPYQGPSRD PDQKGKRNTF QAVLASSDSS SYAIFLYPED GLQFHTTFSK KENNQVPAVV AFSQGSVGFL WKSNGAYNIF ANDRESVENL AKSSNSGQQG VWVFEIGSPA TTNGVVPADV ILGTEDGAEY DDEDEDYDLA TTRLGLEDVG TTPFSYKALR RGGADTYSVP SVLSPRRAAT ERPLGPPTER TRSFQLAVET FHQQHPQVID VDEVEETGVV FSYNTDSRQT CANNRHQCSV H; Positive Samples: LO2, 293T; Cellular Location: basement membrane, extracellular matrix, extracellular space
Target Information
This gene encodes a member of the nidogen family of basement membrane glycoproteins. The protein interacts with several other components of basement membranes, and may play a role in cell interactions with the extracellular matrix.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: enactin; Entactin; NID 1; NID-1; Nidogen (enactin); Nidogen-1; Nidogen-1;
Gene Aliases: NID; NID1
UniProt ID: (Human) P14543
Entrez Gene ID: (Human) 4811
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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