Note: You clicked on an external link, which has been disabled in order to keep your shopping session open.
Nitrated tyrosine residues in bovine pulmonary artery endothelial cells detected with rabbit anti-nitrotyrosine antibody. Fixed and permeabilized bovine pulmonary artery endothelial cells were treated with either degraded peroxynitrite (top panel) or 100 µM peroxynitrite (bottom panel) for five minutes at room temperature to induce protein nitration. Nitrated tyrosine residues were detected with our rabbit anti-nitrotyrosine polyclonal antibody (Cat. No. A21285) and visualized with the green-fluorescent Alexa Fluor® 488 goat anti–rabbit IgG antibody (Cat. No. A11008). Nuclei were counterstained with blue-fluorescent DAPI (Cat. No. D1306, D3571, D21490).
|Tested species reactivity||Chemical|
|Host / Isotype||Rabbit / IgG|
|Immunogen||keyhole limpet hemocyanin (KLH)|
|Storage buffer||PBS, pH 7.2|
|Contains||5mM sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:200-1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Nitrotyrosine is a relatively stable product formed from various reaction pathways. Perhaps most notable is the reaction of peroxynitrite (formed from Superoxide and nitric oxide radicals) with tyrosine. As a strong oxidant and nitrating agent, peroxynitrite mediates tyrosine nitration reactions on proteins resulting in inactivation of certain housekeeping enzymes (e. g. alpha1-antiproteinase) as well as endogenous antioxidant enzymes such as catalase and SOD. Nitrotyrosine has been identified as an indicator of cell damage and inflammation, as well as of the production of NO. It is believed that measuring the concentration of nitrotyrosine will serve as a marker for damage caused by NO in the cell. Nitrotyrosine has been implicated in the pathogenesis of several inflammatory, infectious and degenerative human diseases, such as Alzheimer's disease, amyotrophic lateral sclerosis (ALS), asthma, atherosclerosis and a variety of conditions precipitated by endothelial injury.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.