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|Tested species reactivity||Chemical|
|Host / Isotype||Rabbit / IgG|
|Purification||Ammonium sulfate precipitation|
|Storage buffer||HEPES with 0.15M NaCl, 0.01% BSA, 50% glycerol|
|Contains||0.03% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
A suggested positive control for this product is A431 cell lysate treated with 10mM peroxynitrite.
Nitrotyrosine is a relatively stable product formed from various reaction pathways. Perhaps most notable is the reaction of peroxynitrite (formed from Superoxide and nitric oxide radicals) with tyrosine. As a strong oxidant and nitrating agent, peroxynitrite mediates tyrosine nitration reactions on proteins resulting in inactivation of certain housekeeping enzymes (e. g. alpha1-antiproteinase) as well as endogenous antioxidant enzymes such as catalase and SOD. Nitrotyrosine has been identified as an indicator of cell damage and inflammation, as well as of the production of NO. It is believed that measuring the concentration of nitrotyrosine will serve as a marker for damage caused by NO in the cell. Nitrotyrosine has been implicated in the pathogenesis of several inflammatory, infectious and degenerative human diseases, such as Alzheimer's disease, amyotrophic lateral sclerosis (ALS), asthma, atherosclerosis and a variety of conditions precipitated by endothelial injury.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.