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Immunofluorescent analysis of NuMA in HeLa cells fixed with 3.5% formaldehyde using a NuMA polyclonal antibody (Product # PA1-32451) at a dilution of 1:1000. The staining pattern shows the typical pattern for NuMA in the cell nucleus during interphase and on spindles during mitosis.
|Tested species reactivity||Human, Mammals|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Full-length recombinant human NuMA expressed in insect Sf9 cells using baculovirus expression system, then purified to near homogeneity prior to immunization.|
|Storage buffer||whole serum|
|Contains||0.1% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-32451 detects NuMA from mammal, human samples.
PA1-32451 has been successfully used in immunofluorescence, immunoprecipitation, Western blot applications.
The PA1-32451 immunogen is: Full-length recombinant human NuMA expressed in insect Sf9 cells using baculovirus expression system, then purified to near homogeneity prior to immunization.
NuMA (Nuclear Mitotic Apparatus Protein) is an intranuclear protein and present in nucleus during interphase. At the onset of mitosis, it redistributes from the nucleus to two centrosomal structures that later will become part of the mitotic spindle pole.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
centrophilin stabilizes mitotic spindle in mitotic cells; nuclear matrix protein-22; NUMA; SP-H antigen; structural nuclear protein
NMP-22; NMP22; NUMA; NUMA1