|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgM|
|Immunogen||Synthetic peptide corresponding to residues E(671) S L N K N K H F V P S A G A D S E T H C(691) of human OATP2.|
|Storage buffer||tissue culture supernatant|
|Contains||0.09% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 2 publications below|
MA3-934 detects OATP from human tissue and cells. This antibody does not detect dog, mouse or rat protein. MA3-934 shows no cross-reactivity to other OATP isoforms.
MA3-934 has been successfully used in immunofluorescence, western blotting, and immunoprecipitation procedures. Immunofluorescence staining of OATP2 in human liver samples results in a pattern consistent with plasma membrane staining.
The MA3-934 immunogen is a synthetic peptide corresponding to residues E(671) S L N K N K H F V P S A G A D S E T H C(691) of human OATP2.
The OATPs (organic anion transporter polypeptides) are a family of transport proteins that have been found to mediate the uptake of organic ions into hepatocyte cells. These proteins fall into the solute carrier family 21A (SLC21A), and are expressed in liver, kidney, and brain. These polypeptides are involved in the excretion of potentially toxic compounds and therefore play a role in the overall detoxification system of the body. Unlike other members of the OATP family, OATP2 and 8 have been detected exclusively in the liver, and there is an 80% amino acid sequence homology between these polypeptides. Other names for OATP2 include SLC21A6, OATP-C, and LST-1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Screening of antibiotics that interact with organic anion-transporting polypeptides 1B1 and 1B3 using fluorescent probes.
MA3-934 was used in western blot to identify the antibiotics able to interactg with organic anion-transporting polypeptides 1B1 and 1B3
|Yamaguchi H,Takeuchi T,Okada M,Kobayashi M,Unno M,Abe T,Goto J,Hishinuma T,Shimada M,Mano N||Biological and pharmaceutical bulletin (34:389)||2011|
Transport of fluorescent chenodeoxycholic acid via the human organic anion transporters OATP1B1 and OATP1B3.
MA3-934 was used in western blot to study how the liver uptake of chenodeoxycholic acid is regulated by organic anion-transporting polypeptide (OATP) 1B1 and 1B3
|Yamaguchi H,Okada M,Akitaya S,Ohara H,Mikkaichi T,Ishikawa H,Sato M,Matsuura M,Saga T,Unno M,Abe T,Mano N,Hishinuma T,Goto J||Journal of lipid research (47:1196)||2006|