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OPRK1 Antibody (44-302G) in ICC/IF
Immunofluorescence analysis of kappa-opioid receptor (OPRK1) was performed using 70% confluent log phase PC-12 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with OPRK1 Rabbit Polyclonal Antibody (Product # 44-302G) at 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fl... View More
Product Details
44-302G
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Published species
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
The antiserum was produced against a chemically synthesized peptide derived from the internal region of the mouse k-opioid receptor. The sequence is conserved in rat.
Conjugate
Form
Liquid
Purification
Antigen affinity chromatography
Storage buffer
Dulbecco's PBS, pH 7.3, with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2533627
Target Information
This gene encodes an opioid receptor, which is a member of the 7 transmembrane-spanning G protein-coupled receptor family. It functions as a receptor for endogenous ligands, as well as a receptor for various synthetic opioids. Ligand binding results in inhibition of adenylate cyclase activity and neurotransmitter release. This opioid receptor plays a role in the perception of pain and mediating the hypolocomotor, analgesic and aversive actions of synthetic opioids. Variations in this gene have also been associated with alcohol dependence and opiate addiction. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. A recent study provided evidence for translational readthrough in this gene, and expression of an additional C-terminally extended isoform via the use of an alternative in-frame translation termination codon.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: K-OR-1; Kappa-type opioid receptor; KOPR1; KOR-1; MSL-1; opioid receptor, kappa 2; Ork1
Gene Aliases: K-OR-1; KOR; KOR-1; MSL-1; Oprk1; Oprk2; R21; Ror-d
UniProt ID: (Mouse) P33534, (Rat) P34975
Entrez Gene ID: (Mouse) 18387, (Rat) 29335
immune response
signal transduction
G-protein coupled receptor signaling pathway
G-protein coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger
phospholipase C-activating G-protein coupled receptor signaling pathway
neuropeptide signaling pathway
chemical synaptic transmission
behavior
locomotory behavior
sensory perception of pain
adenylate cyclase-inhibiting opioid receptor signaling pathway
response to insulin
positive regulation of dopamine secretion
negative regulation of luteinizing hormone secretion
opioid receptor signaling pathway
positive regulation of locomotion
maternal behavior
eating behavior
regulation of saliva secretion
behavioral response to cocaine
sensory perception of temperature stimulus
defense response to virus
regulation of sensory perception of pain
positive regulation of p38MAPK cascade
positive regulation of potassium ion transmembrane transport
regulation of aerobic respiration
conditioned place preference
regulation of energy homeostasis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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