Immunofluorescent analysis of OTX2 in immersion-fixed NTera-2 human testicular embryonic carcinoma cells using an OTX2 polyclonal Antibody (Product # PA5-39887) at 10µg/mL for 3 hours at room temperature. Cells were stained using a fluorescent conjugated anti-Goat IgG secondary antibody (yellow, upper panel) and counterstained with DAPI (blue, lower panel).
|Tested species reactivity||Human|
|Host / Isotype||Goat / IgG|
|Immunogen||Recombinant human Otx2, corresponding to Met1-Leu289|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with trehalose|
|Storage Conditions||-20°C or -80°C if preferred|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||5-15 µg/ml|
|Immunofluorescence (IF)||5-15 µg/ml|
|Western Blot (WB)||0.1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
In direct ELISAs, approximately 10% cross-reactivity with recombinant human OTX1 is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
OTX2 is a 289 amino acid containing protein encoding a member of the bicoid sub-family of homeodomain-containing transcription factors. It has two known alternative splice variants with distinct isoforms though presence of other isoforms cannot be excluded. The protein is an early marker of endodermal differentiation of embryonic stem cells and may play a role in the development of the brain and the sensory organs. The protein may bind to BCD target sequence (BTS): 5'-TCTAATCCC-3'. Defects in OTX2 are the cause of microphthalmia syndromic type 5 (MCOPS5).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.