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Invitrogen
ESRRG Polyclonal Antibody
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FIGURE: 1 / 6
ESRRG Antibody (PA1-316) in WB
Western blot analysis of Estrogen-Related Receptor gamma was performed by loading 25 µg of MCF-7 (Lane 1), 293T (Lane 2), and Mouse kidney (Lane 3) cell lysates and a molecular weight protein ladder onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with a blocking buffer at 4°C overnight. The membrane was probed with an Estrogen-Related Receptor gamma polyclonal antibody (Product # PA1-316) at a dilution of 1:500 (MCF-7 and 293T) and 1:1000 (Mouse kidney) overnight at 4°C, washed in TBST, and probed w... View More
Product Details
PA1-316
Applications
Tested Dilution
Publications
Product Specifications
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to residues D(401) Y E A G Q H M E D P R R(413) of human ERR3.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
200 µg
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
Product Specific Information
PA1-316 detects human and mouse ERR3 from transfected cells and shows no cross-reactivity to ERR1.
PA1-316 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~54 kDa protein representing ERR1 from COS-7 transfected cells. This antibody is not suitable for 293T cells in Western blot analysis.
PA1-316 immunogen is a synthetic peptide corresponding to residues D(401) Y E A G Q H M E D P R R(413) of human ERR3. This sequence is completely conserved between human, mouse and rat. This immunizing peptide (PEP-232) can be used for neutralization and control experiments.
Target Information
Estrogen-related receptor gamma (ERRG, ERR3, NR3B3) is an orphan member of the nuclear hormone receptor superfamily. ERRG acts as a transcription activator in the absence of a bound ligand. It binds specifically to an estrogen response element and activates reporter genes controlled by estrogen response elements. ERRG also induces the expression of PERM1 in the skeletal muscle.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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