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Invitrogen
PPARGC1A Polyclonal Antibody
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FIGURE: 1 / 21
PPARGC1A Antibody (PA5-72948) in ICC/IF
Immunocytochemistry analysis of PPARGC1A in HeLa cells. Samples were incubated with PPARGC1A polyclonal antibody (Product # PA5-72948) using a dilution of 1:200 dilution overnight at 4°C followed by anti-rabbit DyLight 488 (Green) at a dilution of 1:500. Cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. Alpha tubulin (DM1A) was used as a co-stain at a 1:1,000 dilution and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with... View More
Product Details
PA5-72948
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Goat,
Hamster,
Human,
Mouse,
Sheep,
Pig,
Rodent,
Rat
Published species
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein made to an internal portion of the human PGC-1 alpha protein (within residues 400-550).
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Expected from sequence similarity: Mouse.
In IHC-P, staining is very strong in the nucleus with some cytoplasmic staining. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Target Information
PGC1 alpha is a transcriptional coactivator that regulates the genes involved in energy metabolism. It interacts with PPARgamma, which permits it's interaction with multiple transcription factors. PGC-1 Alpha can interact with, and regulate the activities of, cAMP response element binding protein (CREB) and nuclear respiratory factors (NRFs). It provides a direct link between external physiological stimuli and the regulation of mitochondrial biogenesis, and is a major factor that regulates muscle fiber type determination. It may be also involved in controlling blood pressure, regulating cellular cholesterol homoeostasis, and the development of obesity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: L-PGC-1alpha; Ligand effect modulator 6; ligand effect modulator-6; peroxisome proliferative activated receptor gamma coactivator 1; peroxisome proliferative activated receptor, gamma, coactivator 1 alpha; peroxisome proliferator activated receptor gamma coactivator 1; peroxisome proliferator activated receptor gamma coactivator-1; peroxisome proliferator activated receptor gamma, coactivator 1 alpha; peroxisome proliferator-activated receptor gamma coactivator 1 alpha; Peroxisome proliferator-activated receptor gamma coactivator 1-alpha; peroxisome proliferator-activated receptor gamma coactivator-1 alpha; peroxisome proliferator-activated receptor gamma, coactivator 1 alpha; pgc-1 alpha; PGC-1-alpha; PGC-1a-like; PGC-1alpha; Pgc-1alphaa; PPAR gamma coactivator 1; PPAR gamma coactivator variant form; PPAR Gamma Coactivator-1; PPAR-gamma coactivator; PPAR-gamma coactivator 1-alpha; PPARAGCIalpha; PPARgamma coactivator 1alpha; PPARGC-1-alpha; transcriptional coactivator; transcriptional coactivator; PGC-1; unnamed protein product
Gene Aliases: A830037N07Rik; ENSMUSG00000079510; Gm11133; I79_015506; LEM6; PGC-1; PGC-1(alpha); PGC-1alpha; PGC-1v; PGC1; PGC1A; Pgco1; PPARGC-1; PPARGC1; PPARGC1A
UniProt ID: (Pig) Q865B6, (Human) Q9UBK2, (Mouse) O70343, (Rat) Q9QYK2
Entrez Gene ID: (Goat) 100861288, (Pig) 397013, (Sheep) 443270, (Dog) 479127, (Human) 10891, (Chinese hamster) 100753651, (Mouse) 19017, (Rat) 83516
DNA binding
transcription coactivator activity
RNA binding
ligand-dependent nuclear receptor binding
chromatin DNA binding
ubiquitin protein ligase binding
sequence-specific DNA binding
nucleic acid binding
transcription cofactor activity
protein binding
RNA polymerase II sequence-specific DNA binding transcription factor binding
lncRNA binding
DNA-binding transcription factor binding
nucleotide binding
RNA polymerase II transcription cofactor activity
chromatin binding
transcription factor binding
ligand-dependent nuclear receptor transcription coactivator activity
transcription cofactor
response to dietary excess
regulation of transcription, DNA-templated
mitochondrion organization
positive regulation of mitochondrion organization
response to muscle activity
respiratory electron transport chain
circadian regulation of gene expression
cellular response to oxidative stress
regulation of circadian rhythm
negative regulation of neuron apoptotic process
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
brown fat cell differentiation
positive regulation of sequence-specific DNA binding transcription factor activity
adipose tissue development
energy homeostasis
positive regulation of cold-induced thermogenesis
negative regulation of neuron death
positive regulation of cellular respiration
positive regulation of mitochondrial DNA metabolic process
positive regulation of muscle tissue development
positive regulation of ATP biosynthetic process
temperature homeostasis
cellular glucose homeostasis
gluconeogenesis
transcription initiation from RNA polymerase II promoter
mRNA processing
digestion
RNA splicing
positive regulation of gene expression
fatty acid oxidation
developmental process
homeostatic process
response to starvation
cellular respiration
positive regulation of gluconeogenesis
positive regulation of fatty acid oxidation
rhythmic process
negative regulation of smooth muscle cell proliferation
protein stabilization
neuron apoptotic process
protein-containing complex assembly
transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
negative regulation of smooth muscle cell migration
positive regulation of cellular metabolic process
negative regulation of glycolytic process
positive regulation of smooth muscle cell proliferation
negative regulation of receptor activity
regulation of N-methyl-D-aspartate selective glutamate receptor activity
positive regulation of energy homeostasis
response to hypoxia
galactose metabolic process
androgen metabolic process
response to cold
response to organic cyclic compound
response to activity
response to nutrient levels
response to statin
response to drug
response to leucine
flavone metabolic process
cellular response to nitrite
cellular response to tumor necrosis factor
cellular response to fatty acid
cellular response to hypoxia
response to epinephrine
response to norepinephrine
cellular response to thyroid hormone stimulus
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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