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Invitrogen

Phospho-PAK1/2/3 (Ser144, Ser141, Ser139) Polyclonal Antibody

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Phospho-PAK1/2/3 (Ser144, Ser141, Ser139) Polyclonal Antibody

Product Details

PA5-101019

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000-1:3,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

Assay-dependent
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human PAK3(Accession O75914), corresponding to amino acid residues around phosphorylated Ser139.
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Amount

100 µg

Purification

sequential chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2815853

Product Specific Information

Antibody detects endogenous levels of PAK1/2/3 only when phosphorylated at PS144+S141+S139.

Target Information

In mammals, there are several identified isoforms of p21-Activated Protein Kinases or PAKs: alpha-PAK (also known as PAK-1) and beta-PAK (also known as PAK-3) are mostly brain-specific, while gamma-PAK (also known as PAK-2) is expressed ubiquitously (Jakobi et al., 2003). Mutations of the gene coding for PAK-3 are associated wit X-linked mental retardation and recent work indicates that PAK-3 is a key regulator of synapse formation and plasticity in the hippocampus (Boda et al., 2004). PAK-3 is thought to play a key role in regulation of cell shape and motility as well as cell death (Jakobi et al., 2003; Walter et al., 1998). Autophosphorylation of Thr402 in the protein has been found to be essential for activation of PAK (Jakobi et al., 2000).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: activated protein kinase alpha; ADRB2; adriamycin resistance-associated; Alpha-PAK; aPAK; Beta-PAK; BPAK; CDC42/RAC effector kinase PAK-A; CDC42/RAC effector kinase PAK-B; CDKN1A; CLAP; EC 2.7.11.1; gamma pak; Gamma-PAK; hPAK3; kinase PAK1; Oligophrenin-3; p21 (CDKN1A)-activated kinase 1; p21 (CDKN1A)-activated kinase 2; p21 (CDKN1A)-activated kinase 3; p21 protein (Cdc42/Rac)-activated kinase 1; p21 protein (Cdc42/Rac)-activated kinase 2; p21 protein (Cdc42/Rac)-activated kinase 3; p21-activated kinase 1; p21-activated kinase 2; p21-activated kinase 3; p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast); p21/Cdc42/Rac1-activated kinase 1 (yeast Ste20-related); p21/Cdc42/Rac1-activated kinase 3 (yeast Ste20-related); p58; p65-PAK; p68-PAK; PAK 1; PAK alpha; PAK beta; PAK gamma; PAK-1; PAK-2; PAK-3; PAK-a; PAK-B; PAK-y; PAK65; protein kinase; Protein kinase MUK2; S6/H4 kinase; Serine/threonine-protein kinase PAK 1; Serine/threonine-protein kinase PAK 2; Serine/threonine-protein kinase PAK 3; STE20 homolog, yeast; unnamed protein product; yPAK

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Gene Aliases: 5330420P17Rik; A130002K10Rik; AI836325; alpha-PAK; ARA; AW045634; beta-PAK; bPAK; D16Ertd269e; gamma-PAK; IDDMSSD; KNO2; mKIAA4182; MRX30; MRX47; OPHN3; p65-PAK; PAK-1; PAK-2; PAK-3; PAK1; PAK2; PAK3; PAK3beta; PAK65; Pak65alpha; Pak65beta; Paka; PAKalpha; Pakb; PAKgamma; Stk4; XLID30

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UniProt ID: (Human) Q13153, (Human) Q13177, (Human) O75914, (Rat) P35465, (Mouse) Q8CIN4, (Rat) Q64303, (Mouse) Q61036, (Rat) Q62829

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Entrez Gene ID: (Human) 5058, (Human) 5062, (Human) 5063, (Mouse) 18479, (Rat) 29431, (Mouse) 224105, (Rat) 29432, (Mouse) 18481, (Rat) 29433

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Function(s)
nucleotide binding catalytic activity protein kinase activity protein serine/threonine kinase activity protein binding collagen binding ATP binding kinase activity transferase activity protein kinase binding small GTPase binding identical protein binding gamma-tubulin binding protein serine kinase activity protein tyrosine kinase activator activity cadherin binding MAP kinase kinase activity SH3 domain binding metal ion binding transferase activity, transferring phosphorus-containing groups Rac GTPase binding MAP kinase kinase kinase activity receptor signaling protein serine/threonine kinase activity Rho GTPase binding GTPase binding non-receptor serine/threonine protein kinase
Process(es)
MAPK cascade response to hypoxia stimulatory C-type lectin receptor signaling pathway chromatin remodeling exocytosis apoptotic process cellular response to DNA damage stimulus transmembrane receptor protein tyrosine kinase signaling pathway positive regulation of cell proliferation cellular response to starvation positive regulation of fibroblast migration cell migration cerebellum development establishment of cell polarity actin cytoskeleton organization positive regulation of cell migration positive regulation of microtubule polymerization cellular response to insulin stimulus regulation of actin cytoskeleton organization positive regulation of intracellular estrogen receptor signaling pathway intracellular signal transduction Fc-gamma receptor signaling pathway involved in phagocytosis wound healing regulation of MAPK cascade positive regulation of axon extension positive regulation of insulin receptor signaling pathway hepatocyte growth factor receptor signaling pathway ephrin receptor signaling pathway branching morphogenesis of an epithelial tube neuron projection morphogenesis regulation of axonogenesis positive regulation of stress fiber assembly negative regulation of cell proliferation involved in contact inhibition negative regulation of cell growth involved in cardiac muscle cell development positive regulation of microtubule nucleation positive regulation of protein targeting to membrane regulation of actin filament organization protein localization to cytoplasmic stress granule positive regulation of vascular smooth muscle cell proliferation positive regulation of vascular associated smooth muscle cell migration cardiac muscle hypertrophy protein phosphorylation negative regulation of protein kinase activity signal transduction adherens junction assembly negative regulation of apoptotic process protein autophosphorylation vascular endothelial growth factor receptor signaling pathway regulation of cytoskeleton organization negative regulation of stress fiber assembly protein maturation dendritic spine development bicellular tight junction assembly cellular response to transforming growth factor beta stimulus execution phase of apoptosis protein localization to cell-cell junction positive regulation of execution phase of apoptosis positive regulation of extrinsic apoptotic signaling pathway axonogenesis dendrite development regulation of actin filament polymerization synapse organization dendritic spine morphogenesis regulation of postsynapse organization positive regulation of protein phosphorylation Rho protein signal transduction regulation of mitotic cell cycle neuromuscular junction development metabolic process response to organic substance regulation of gene expression phosphorylation amygdala development signal transduction by protein phosphorylation stress-activated protein kinase signaling cascade actin cytoskeleton reorganization activation of protein kinase activity positive regulation of peptidyl-serine phosphorylation regulation of apoptotic process receptor clustering positive regulation of JUN kinase activity gamma-aminobutyric acid secretion, neurotransmission glutamate secretion, neurotransmission cellular response to organic cyclic compound observational learning regulation of long-term synaptic potentiation activation of MAPKK activity mitotic cell cycle small GTPase mediated signal transduction peptidyl-serine phosphorylation regulation of growth positive regulation of peptidyl-tyrosine phosphorylation positive regulation of protein tyrosine kinase activity negative regulation of cysteine-type endopeptidase activity involved in execution phase of apoptosis activation of MAPK activity multicellular organism development regulation of neuron projection development positive regulation of neuron apoptotic process positive regulation of dendritic spine morphogenesis positive regulation of DNA biosynthetic process
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