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PABPC1/PABP Polyclonal Antibody

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PABPC1/PABP Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of MCF-7 cells, using PABPC1 antibody 10970-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

PABPC1/PABP Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human pancreas using 10970-1-AP (PABPC1,PABP antibody) at dilution of 1:50 (under 10x lens).

PABPC1/PABP Antibody in Western Blot (WB) — Mouse testis tissue were subjected to SDS PAGE followed by western blot with 10970-1-AP (PABPC1,PABP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

PABPC1/PABP Antibody in Flow Cytometry (Flow) — 1X10^6 MCF-7 cells were stained with 0.2ug PABPC1,PABP antibody (10970-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.

PABPC1/PABP Antibody in Immunoprecipitation (IP) — IP result of anti-PABPC1,PABP (IP:10970-1-AP, 4ug; Detection:10970-1-AP 1:700) with mouse testis tissue lysate 4000ug.

Product Details

10970-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	1:20-1:200	-
Immunocytochemistry (ICC/IF)	1:10-1:100	View 1 publication 1 publication
Flow Cytometry (Flow)	0.2 µg/1x10^6 cells	-
Immunoprecipitation (IP)	0.5-4.0 µg	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	PABPC1,PABP Fusion Protein Ag1422 (287-636 aa encoded by BC015958) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.33 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: RITRYQGVN LYVKNLDDGI DDERLRKEFS PFGTITSAKV MMEGGRSKGF GFVCFSSPEE ATKAVTEMNG RIVATKPLYV ALAQRKEERQ AHLTNQYMQR MASVRAVPNP VINPYQPAPP SGYFMAAIPQ TQNRAAYYPP SQIAQLRPSP RWTAQGARPH PFQNMPGAIR PAAPRPPFST MRPASSQVPR VMSTQRVANT STQTMGPRPA AAAAAATPAV RTVPQYKYAA GVRNPQQHLN AQPQVTMQQP AVHVQGQEPL TASMLASAPP QEQKQMLGER LFPLIQAMHP TLAGKITGML LEIDNSELLH MLESPESLRS KVDEAVAVLQ AHQAKEAAQK AVNSATGVPT V (287-636 aa encoded by BC015958)

Target Information

The poly(A)-binding protein (PABP), which is found complexed to the 3-prime poly(A) tail of eukaryotic mRNA, is required for poly(A) shortening and translation initiation. In humans, the PABPs comprise a small nuclear isoform and a conserved gene family that displays at least 3 functional proteins: PABP1 (PABPC1), inducible PABP (iPABP, or PABPC4), and PABP3 (PABPC3). In addition, there are at least 4 pseudogenes, PABPCP1 to PABPCP4.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

TDP-43 aggregation mirrors TDP-43 knockdown, affecting the expression levels of a common set of proteins.

Scientific reports

Prpar Mihevc S,Baralle M,Buratti E,Rogelj B

Mon Sep 26 00:00:00 EDT 2016

TDP-43 aggregation mirrors TDP-43 knockdown, affecting the expression levels of a common set of proteins.

Scientific reports

Prpar Mihevc S,Baralle M,Buratti E,Rogelj B

Mon Sep 26 00:00:00 EDT 2016

Bioinformatics

Protein Aliases: PABP 1; PABP-1; poly A binding protein 1; poly(A) binding protein, cytoplasmic 1; poly(A) binding protein, cytoplasmic 2; poly(A)-binding protein 1; Polyadenylate-binding protein 1

Gene Aliases: ePAB; PAB1; PABP; PABP1; PABPC1; PABPC2; PabpI; PABPL1

UniProt ID: (Human) P11940, (Rat) Q9EPH8, (Mouse) P29341

Entrez Gene ID: (Human) 26986, (Rat) 171350, (Mouse) 18458

Function(s)

nuclear-transcribed mRNA catabolic process, nonsense-mediated decay mRNA processing RNA splicing gene silencing by RNA positive regulation of nuclear-transcribed mRNA poly(A) tail shortening positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay negative regulation of nuclear-transcribed mRNA catabolic process, nonsense-mediated decay nuclear-transcribed mRNA poly(A) tail shortening mRNA splicing, via spliceosome mRNA polyadenylation translational initiation regulation of mRNA stability positive regulation of viral genome replication positive regulation of translation mRNA stabilization

Process(es)

nucleotide binding protein binding protein C-terminus binding poly(A) binding poly(U) RNA binding translation activator activity poly(A) RNA binding mRNA binding nucleic acid binding RNA binding

PABPC1/PABP Polyclonal Antibody

Product Details

10970-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Product Specific Information

Target Information

Bioinformatics

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