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PAK1IP1 Antibody (PA5-100854) in ICC/IF
Immunocytochemistry analysis of PAK1IP1 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with PAK1IP1 polyclonal antibody (Product # PA5-100854) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-100854
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human PAK1IP1(Accession Q9NWT1), corresponding to amino acid residues E325-M375.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2850352
Product Specific Information
Antibody detects endogenous levels of total PAK1IP1.
Target Information
Negatively regulates the PAK1 kinase. PAK1 is a member of the PAK kinase family, which has been shown to play a positive role in the regulation of signaling pathways involving MAPK8 and RELA. PAK1 exists as an inactive homodimer, which is activated by binding of small GTPases such as CDC42 to an N-terminal regulatory domain. PAK1IP1 also binds to the N-terminus of PAK1, and inhibits the specific activation of PAK1 by CDC42. May be involved in ribosomal large subunit assembly (PubMed:24120868). [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: glycerophosphodiester phosphodiesterase domain containing 1; hPIP1; p21-activated protein kinase-interacting protein 1; PAK/PLC-interacting protein 1; PAK1-interacting protein 1; Putative PAK inhibitor Skb15; RP11-421M1.5; WD repeat-containing protein 84
Gene Aliases: 5830431I15Rik; 5930415H02Rik; AA419825; AI314040; AW556169; bA421M1.5; Gdpd1; hPIP1; MAK11; PAK1IP1; PIP1; WDR84
UniProt ID: (Human) Q9NWT1, (Mouse) Q9DCE5
Entrez Gene ID: (Human) 55003, (Mouse) 68083
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