Bioss

PAPOA Polyclonal Antibody

View all (7) PAPOLA antibodies

Questions & Answers

Questions & Answers

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PAPOA Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Formalin-fixed and paraffin embedded rat brain labeled with Anti-PAPOA Polyclonal Antibody, Unconjugated (bs-4966R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

PAPOA Antibody in ELISA (ELISA) — Antigen: bs-4966P, 0.2ug/100ul Primary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1:5000; TMB staining; Read the data in Microplate Reader by 450nm.

Product Details

BS-4966R

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:200	-
Immunohistochemistry (Frozen) (IHC (F))	1:100-1:500	-
Immunocytochemistry (ICC/IF)	1:100-1:500	-
ELISA (ELISA)	1:5,000-1:10,000	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	KLH conjugated synthetic peptide derived from human PAPOA, amino acids 601-700. View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Protein A
Storage buffer	PBS with 50% glycerol, 1% BSA
Contains	0.09% sodium azide
Storage conditions	-20°C

Target Information

Polymerase that creates the 3'-poly(A) tail of mRNA's. Also required for the endoribonucleolytic cleavage reaction at some polyadenylation sites. May acquire specificity through interaction with a cleavage and polyadenylation specificity factor (CPSF) at its C-terminus.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: PAP-alpha; Poly(A) polymerase alpha; Polynucleotide adenylyltransferase; Polynucleotide adenylyltransferase alpha

Gene Aliases: PAP; PapIII; PAPOLA; Plap

UniProt ID: (Human) P51003, (Mouse) Q61183

Entrez Gene ID: (Human) 10914, (Mouse) 18789, (Rat) 314417

Molecular Function: mRNA polyadenylation factor

mRNA splicing, via spliceosome termination of RNA polymerase II transcription mRNA polyadenylation mRNA 3'-end processing regulation of mRNA 3'-end processing RNA polyadenylation mRNA processing RNA 3'-end processing biological_process

Process(es)

magnesium ion binding RNA binding polynucleotide adenylyltransferase activity protein binding ATP binding manganese ion binding nucleotide binding transferase activity nucleotidyltransferase activity metal ion binding molecular_function