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PARD3 Polyclonal Antibody

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PARD3 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ( -20°C Ethanol ) fixed MCF-7 cells using 11085-1-AP (PARD3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

PARD3 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human colon cancer using 11085-1-AP (PARD3 antibody) at dilution of 1:50 (under 40x lens).

PARD3 Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA)) — Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using 11085-1-AP (PARD3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

PARD3 Antibody in Western Blot (WB) — MCF7 cells were subjected to SDS PAGE followed by western blot with 11085-1-AP (PARD3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

PARD3 Antibody in Flow Cytometry (Flow) — 1X10^6 MCF-7 cells were stained with 0.2ug PARD3 antibody (11085-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.

PARD3 Antibody in Immunoprecipitation (IP) — IP result of anti-PARD3 (IP:11085-1-AP, 4ug; Detection:11085-1-AP 1:200) with MCF-7 cells lysate 2900ug.

Product Details

11085-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:1,000	View 2 publications 2 publications
Immunohistochemistry (IHC)	-	View 3 publications 3 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:200	-
Immunohistochemistry (PFA fixed) (IHC (PFA))	1:50-1:500	-
Immunocytochemistry (ICC/IF)	1:50-1:500	View 2 publications 2 publications
Flow Cytometry (Flow)	0.2 µg/1x10^6 cells	-
Immunoprecipitation (IP)	0.5-4.0 µg	-
Miscellaneous PubMed (Misc)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Chicken, Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	PARD3 Fusion Protein Ag1565 (217-497 aa encoded by BC011711) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.14 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

PARD3 has three splice isoforms at 100 kDa, 150 kDa, and 180 kDa. This polyclonal antibody raised against C-terminal 281 amino acids of human PARD3 recognizes these three isoforms.

Immunogen sequence: RERDYAEIQ DFHRTFGCDD ELMYGGVSSY EGSMALNARP QSPREGHMMD ALYAQVKKPR NSKPSPVDSN RSTPSNHDRI QRLRQEFQQA KQDEDVEDRR RTYSFEQPWP NARPATQSGR HSVSVEVQMQ RQRQEERESS QQAQRQYSSL PRQSRKNASS VSQDSWEQNY SPGEGFQSAK ENPRYSSYQG SRNGYLGGHG FNARVMLETQ ELLRQEQRRK EQQMKKQPPS EGPSNYDSYK KVQDPSYAPP KGPFRQDVPP SPSQVARLNR LQTPEKGRPF YS (217-497 aa encoded by BC011711)

Target Information

This gene encodes a member of the kinesin 4 or subfamily of kinesin related proteins. This subfamily is also referred to as the chromokinesins. The encoded protein is an ATP dependent microtubule-based motor protein that is involved in the intracellular transport of membranous organelles. This protein also associates with condensed chromosome arms and may be involved maintaining chromosome integrity during mitosis. This protein may also be involved in the organization of the central spindle prior to cytokinesis. A pseudogene of this gene is found on chromosome X.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

AhR activation protects intestinal epithelial barrier function through regulation of Par-6.

Journal of molecular histology

Yu K,Ma Y,Zhang Z,Fan X,Li T,Li L,Xiao W,Cai Y,Sun L,Xu P,Yu M,Yang H

Mon Oct 01 00:00:00 EDT 2018

Perturbation of epithelial apicobasal polarity by rhomboid family-1 gene overexpression.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Peng XM,Gao S,Deng HT,Cai HX,Zhou Z,Xiang R,Zhang QZ,Li LY

Mon Oct 01 00:00:00 EDT 2018

Coupling of apical-basal polarity and planar cell polarity to interpret the Wnt signaling gradient in feather development.

Development (Cambridge, England)

Lin J,Yue Z

Fri Sep 14 00:00:00 EDT 2018

Deletion of Brg1 causes abnormal hair cell planer polarity, hair cell anchorage, and scar formation in mouse cochlea.

Scientific reports

Jin Y,Ren N,Li S,Fu X,Sun X,Men Y,Xu Z,Zhang J,Xie Y,Xia M,Gao J

Fri Jun 03 00:00:00 EDT 2016

Par-3 modulates intestinal epithelial barrier function through regulating intracellular trafficking of occludin and myosin light chain phosphorylation.

Journal of gastroenterology

Yu M,Yang S,Qiu Y,Chen G,Wang W,Xu C,Cai W,Sun L,Xiao W,Yang H

Sun Nov 01 00:00:00 EDT 2015

Par-3 modulates intestinal epithelial barrier function through regulating intracellular trafficking of occludin and myosin light chain phosphorylation.

Journal of gastroenterology

Yu M,Yang S,Qiu Y,Chen G,Wang W,Xu C,Cai W,Sun L,Xiao W,Yang H

Sun Nov 01 00:00:00 EDT 2015

Overexpression of EphB4 in the mammary epithelium shifts the differentiation pathway of progenitor cells and promotes branching activity and vascularization.

Development, growth & differentiation

Kaenel P,Hahnewald S,Wotzkow C,Strange R,Andres AC

Thu May 01 00:00:00 EDT 2014

Transgenic expression of constitutively active RAC1 disrupts mouse rod morphogenesis.

Investigative ophthalmology & visual science

Song H,Bush RA,Vijayasarathy C,Fariss RN,Kjellstrom S,Sieving PA

Fri Apr 25 00:00:00 EDT 2014

Deregulated ephrin-B2 signaling in mammary epithelial cells alters the stem cell compartment and interferes with the epithelial differentiation pathway.

International journal of oncology

Kaenel P,Antonijevic M,Richter S,Küchler S,Sutter N,Wotzkow C,Strange R,Andres AC

Wed Feb 01 00:00:00 EST 2012

Bioinformatics

Protein Aliases: ASBP; ASIP; atypical PKC isotype-specific interacting protein; Atypical PKC isotype-specific-interacting protein; atypical PKC-specific binding protein; Atypical PKC-specific-binding protein; bazooka; CTCL tumor antigen se2-5; Ephrin-interacting protein; FLJ21015; PAR-3; par-3 (partitioning defective 3) homolog; par-3 family cell polarity regulator alpha; par-3 partitioning defective 3 homolog; PAR3-alpha; PARD-3; Partitioning defective 3 homolog; partitioning-defective 3 homolog; PHIP; protein phosphatase 1, regulatory subunit 118; RP11-406D17.1; three-PDZ containing protein similar to C. elegans PAR3 (partitioning defect)

Gene Aliases: AA960621; AI256638; ASIP; Baz; D8Ertd580e; Par-3; PAR3; PAR3A; PAR3alpha; PARD-3; PARD3; PARD3A; Phip; PPP1R118; SE2-5L16; SE2-5LT1; SE2-5T2

UniProt ID: (Human) Q8TEW0, (Mouse) Q99NH2, (Rat) Q9Z340

Entrez Gene ID: (Human) 56288, (Mouse) 93742, (Rat) 81918

Function(s)

microtubule cytoskeleton organization apical constriction protein targeting to membrane cell cycle establishment or maintenance of cell polarity negative regulation of peptidyl-threonine phosphorylation single organismal cell-cell adhesion myelination in peripheral nervous system cell differentiation positive regulation of myelination regulation of actin filament-based process wound healing, spreading of cells cell division centrosome localization regulation of cellular localization bicellular tight junction assembly establishment of epithelial cell polarity protein complex assembly transforming growth factor beta receptor signaling pathway protein kinase C-activating G-protein coupled receptor signaling pathway axonogenesis asymmetric cell division establishment or maintenance of epithelial cell apical/basal polarity

Process(es)

protein binding phosphatidylinositol-4,5-bisphosphate binding phosphatidylinositol-3,4,5-trisphosphate binding protein phosphatase binding phosphatidylinositol-3-phosphate binding protein kinase C binding lipid binding

PARD3 Polyclonal Antibody

Product Details

11085-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Product Specific Information

Target Information

Bioinformatics

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