Western blot analysis of HeLa Nuclear Lysate using PA5-19791, PARD3 primary antibody at a dilution of 1 ug/ml (lane 1). PA5-19791 staining of SW480 Whole Cell Lysate at a dilution of 1 ug/ml (lane 2). Blot treated with a secondary HRP-conjugated Goat polyclonal anti-Rabbit antibody was used at a dilution of 1:3000.
|Tested species reactivity||Human, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide conjugated to KLH derived from within residues 1300 to the C-terminus of Human PARD3.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||1-5 µg/ml|
|Western Blot (WB)||1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
For Western Blot, this antibody has non-specific bands at 170 kDa,45 kDa,53 kDa and 60 kDa.
This antibody is predicted to react with mouse, sheep and chimpanzee based on sequence homology.
Store antibody at 4°C for 1-2 weeks. For long-term storage, store at -20°C.
PARD proteins, which were first identified in C. elegans, are essential for asymmetric cell division and polarized growth, whereas CDC42 mediates the establishment of cell polarity. The CDC42 GTPase, which is controlled by nucleotide exchange factors and GTPase-activating proteins domain.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.