Note: You clicked on an external link, which has been disabled in order to keep your shopping session open.
Immunofluorescence was performed on mouse E10.5 embryo trunk at the forelimb level. Embryos were fixed with 4%PFA in 0.1M phosphate buffer, pH 7.2 at 4C for 30 minutes, processed for cryoprotection in 20% sucrose, and embedded in OCT compound for cryosectioning at 10 um thickness. Following sectioning, tissues were washed in PBS with 0.1% Triton-X 100 (PBT), blocked in 1% goat serum in PBT for 30 minutes at room temperature, and then probed with a Pax3 polyclonal antibody (Product # PA1-107) at a concentration of 1ug/ml in blocking buffer overnight at 4C. Tissues were washed extensively with PBT, and detection was performed using an Alexa Fluor 568-conjugated goat anti-rabbit IgG secondary antibody (red) at a dilution of 1:1000 in blocking buffer. Nuclei (blue) were stained with DAPI. Note: Staining was observed in the dorsal neural tube (symmetric two halves on the left side of the image), the dermomyotome/myotome (middle streak between symmetric halves), and limb muscle progenitors (scattered cells to the right side of the image). Data supplied courtesy of the Innovator's Program.
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Amino acids 301-442 of human PAX3|
|Storage buffer||PBS with 1mg/ml BSA, 30% glycerol|
|Contains||0.05% sodium azide|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||1:100-1:1000|
|Western Blot (WB)||1:500-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-107 has successfully been used in Western blot for detecting overexpressed and recombinant human Pax3 protein, but may not be suitable for detecting endogenous Pax3.
By immunofluorescence, PA1-107 has been used to stain frozen embryonic mouse brain tissue.
PA1-107 has been validated for IP using overexpressed Pax3 protein.
Pax 3 (Paired Box 3) is a transcription factor that is involved in development of the peripheral nervous system, melanocytes, some vascular smooth muscle, and a number of other derivatives. It regulates neurogenesis in pre-migratory neural crest cells from the dorsal neural tube, and in myogenic progenitors in the presomitic mesoderm and the hypaxial somites. Alternative splicing results in isoforms having different C-termini.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
CDHS; HUP2; Paired Box 3; Paired Box Gene 3; Paired Box Homeotic Gene 3; paired box protein Pax-3; Paired Box Protein-3; Paired Domain Gene 3; Paired Domain Gene HuP2; PAX3/FKHR fusion; Waardenburg Syndrome 1; WS1; WS3
CDHS; HUP2; Pax-3; PAX3; Sp; splotch; WS1; WS3