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Invitrogen
PC1/3 Polyclonal Antibody
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FIGURE: 1 / 3
PC1/3 Antibody (PA5-79787) in IHC (P)
Immunohistochemical analysis of PC1/3/PCSK1 in paraffin-embedded section of human colon cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti-PC1/3/PCSK1 antibody (Product # PA5-79787) overnight at 4°C. Biotin conjugated go... View More
Product Details
PA5-79787
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human PC1/3 (726-740aa DVFYNTKPYKHRDDR).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide, 0.05mg thimerosal
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: Rat Liver Tissue, Rat Thymus Tissue, A549 Cell, HELA Cell, COLO320 Cell, PANC Cell. IHC: rat intestine tissue.
Target Information
PCSK1 (Proprotein Convertase, Subtilisin/Kexin-Type, 1), also known as PC1 or NEC1, is an enzyme that in humans is encoded by the PCSK1 gene. Proprotein convertase-1 is a neuroendocrine convertase that belongs to a family of subtilisin-like serine endoproteases that process large precursor proteins into mature bioactive products. By in situ hybridization, Seidah et al. (1991) mapped the NEC1 gene to human chromosome 5q15-q21 and mouse chromosome 13. Ohagi et al. (1996) noted that PC1 initiates the sequential processing of proinsulin to insulin by cleaving the proinsulin molecule on the C-terminal side of the dibasic peptide, arg31-arg32, joining the B-chain and C-peptide. By observing the phenotypic features in patients with PC1 mutations, Jackson et al. (2003) concluded that human intestinal absorptive function is dependent on PC1 activity.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Furin homolog; NEC 1; Neuroendocrine convertase 1; PC1; PC3; Prohormone convertase 1; prohormone convertase 1/3; prohormone convertase 3; Propeptide-processing protease; Proprotein convertase 1; proprotein convertase 1/3; Protein convertase subtilisin / kexin type I; Protein convertase subtilisin / kexin, type I; unnamed protein product
Gene Aliases: Att-1; BDP; BMIQ12; Nec-1; NEC1; PC1; PC1/3; PC3; PCSK1; Phpp-1; SPC3
UniProt ID: (Human) P29120, (Rat) P28840, (Mouse) P63239
Entrez Gene ID: (Human) 5122, (Rat) 25204, (Mouse) 18548
proteolysis
cell-cell signaling
protein processing
peptide hormone processing
insulin processing
peptide biosynthetic process
response to glucose
response to inorganic substance
response to chlorate
response to organic cyclic compound
protein autoprocessing
pituitary gland development
neurogenesis
pancreas development
response to nutrient levels
response to lipopolysaccharide
response to drug
response to morphine
response to peptide hormone
response to axon injury
positive regulation of protein secretion
response to glucocorticoid
response to calcium ion
response to fatty acid
response to interleukin-1
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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