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PCAF Antibody (PA5-22046) in WB
Western Blot analysis of PCAF was performed by separating 30 µg of various whole cell lysates by 7.5 % SDS-PAGE. Proteins were transferred to a membrane and probed with a PCAF Polyclonal Antibody (Product # PA5-22046) at a dilution of 1:1000. A. 293T, B. A431, C. A375.
Product Details
PA5-22046
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to a region within amino acids 767 and 832 of PCAF (Uniprot ID#Q92831)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_11152160
Product Specific Information
Recommended positive controls: 293T, A431, A375, HeLa, HeLa nuclear extract, MFB.
Predicted reactivity: Mouse (100%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
PCAF (histone acetyltransferase KAT2B) functions as a histone acetyltransferase (HAT) to promote transcriptional activation. It has significant histone acetyltransferase activity with core histones (H3 and H4) and nucleosome core particles. PCAF also acetylates non-histone proteins such as ACLY, PLK4, and TBX5. It inhibits cell-cycle progression and counteracts the mitogenic activity of the adenoviral oncoprotein E1A. PCAF also acts as a circadian transcriptional coactivator which enhances the activity of the circadian transcriptional activators NPAS2-ARNTL/BMAL1 and CLOCK-ARNTL/BMAL1 heterodimers. It is involved in heart and limb development by mediating acetylation of TBX5 and acetylation regulating nucleocytoplasmic shutting of TBX5.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CREBBP-associated factor; Histone acetylase PCAF; Histone acetyltransferase KAT2B; Histone acetyltransferase PCAF; K(lysine) acetyltransferase 2B; Lysine acetyltransferase 2B; P/ PCAF; P/CAF; P300/CBP-associated factor; Spermidine acetyltransferase KAT2B
Gene Aliases: A930006P13Rik; AI461839; AW536563; CAF; KAT2B; P/CAF; PCAF
UniProt ID: (Human) Q92831, (Mouse) Q9JHD1
Entrez Gene ID: (Human) 8850, (Mouse) 18519
RNA polymerase II regulatory region sequence-specific DNA binding
chromatin binding
transcription cofactor activity
transcription coactivator activity
histone acetyltransferase activity
lysine N-acetyltransferase activity, acting on acetyl phosphate as donor
cyclin-dependent protein serine/threonine kinase inhibitor activity
protein binding
transcription factor binding
acetyltransferase activity
protein kinase binding
protein complex binding
histone acetyltransferase binding
histone deacetylase binding
N-acetyltransferase activity
transferase activity
transferase activity, transferring acyl groups
chromatin remodeling
transcription initiation from RNA polymerase II promoter
protein acetylation
cell cycle arrest
Notch signaling pathway
memory
negative regulation of cell proliferation
regulation of protein ADP-ribosylation
positive regulation of neuron projection development
viral process
N-terminal peptidyl-lysine acetylation
internal peptidyl-lysine acetylation
peptidyl-lysine acetylation
cellular response to insulin stimulus
cellular response to oxidative stress
positive regulation of chromatin binding
positive regulation of gluconeogenesis by positive regulation of transcription from RNA polymerase II promoter
histone H3 acetylation
histone H3-K9 acetylation
negative regulation of cyclin-dependent protein serine/threonine kinase activity
positive regulation of gene expression, epigenetic
positive regulation of vasodilation
positive regulation of transcription from RNA polymerase II promoter
rhythmic process
cellular response to parathyroid hormone stimulus
positive regulation of histone H3-K14 acetylation
positive regulation of histone H3-K9 acetylation
transcription, DNA-templated
regulation of transcription, DNA-templated
cell cycle
histone acetylation
positive regulation of transcription, DNA-templated
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