Invitrogen

PCAF Polyclonal Antibody

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Datasheet
Protocols
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Datasheet
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Tech Support
PCAF Polyclonal Antibody

Product Details

PA5-99179

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:3,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-

Immunocytochemistry (ICC/IF)

1:100-1:500
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human KAT2B(Accession Q92831), corresponding to amino acid residues K800-K832.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2818112

Product Specific Information

Antibody detects endogenous levels of total PCAF.

Target Information

PCAF (histone acetyltransferase KAT2B) functions as a histone acetyltransferase (HAT) to promote transcriptional activation. It has significant histone acetyltransferase activity with core histones (H3 and H4) and nucleosome core particles. PCAF also acetylates non-histone proteins such as ACLY, PLK4, and TBX5. It inhibits cell-cycle progression and counteracts the mitogenic activity of the adenoviral oncoprotein E1A. PCAF also acts as a circadian transcriptional coactivator which enhances the activity of the circadian transcriptional activators NPAS2-ARNTL/BMAL1 and CLOCK-ARNTL/BMAL1 heterodimers. It is involved in heart and limb development by mediating acetylation of TBX5 and acetylation regulating nucleocytoplasmic shutting of TBX5.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CREBBP-associated factor; Histone acetylase PCAF; Histone acetyltransferase KAT2B; Histone acetyltransferase PCAF; K(lysine) acetyltransferase 2B; Lysine acetyltransferase 2B; P/ PCAF; P/CAF; P300/CBP-associated factor; Spermidine acetyltransferase KAT2B

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Gene Aliases: A930006P13Rik; AI461839; AW536563; CAF; KAT2B; P/CAF; PCAF

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UniProt ID: (Human) Q92831, (Mouse) Q9JHD1

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Entrez Gene ID: (Human) 8850, (Rat) 301164, (Mouse) 18519

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Function(s)
RNA polymerase II regulatory region sequence-specific DNA binding chromatin binding transcription cofactor activity transcription coactivator activity histone acetyltransferase activity lysine N-acetyltransferase activity, acting on acetyl phosphate as donor cyclin-dependent protein serine/threonine kinase inhibitor activity protein binding transcription factor binding acetyltransferase activity protein kinase binding protein complex binding histone acetyltransferase binding histone deacetylase binding N-acetyltransferase activity transferase activity transferase activity, transferring acyl groups
Process(es)
chromatin remodeling protein acetylation memory negative regulation of cell proliferation regulation of protein ADP-ribosylation positive regulation of neuron projection development histone acetylation N-terminal peptidyl-lysine acetylation internal peptidyl-lysine acetylation peptidyl-lysine acetylation cellular response to insulin stimulus cellular response to oxidative stress positive regulation of chromatin binding positive regulation of gluconeogenesis by positive regulation of transcription from RNA polymerase II promoter histone H3 acetylation histone H3-K9 acetylation negative regulation of cyclin-dependent protein serine/threonine kinase activity positive regulation of transcription, DNA-templated positive regulation of vasodilation positive regulation of transcription from RNA polymerase II promoter cellular response to parathyroid hormone stimulus positive regulation of histone H3-K14 acetylation positive regulation of histone H3-K9 acetylation transcription initiation from RNA polymerase II promoter cell cycle arrest Notch signaling pathway viral process positive regulation of gene expression, epigenetic rhythmic process transcription, DNA-templated regulation of transcription, DNA-templated cell cycle
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