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Invitrogen
PCBP2 Polyclonal Antibody
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FIGURE: 1 / 3
PCBP2 Antibody (PA5-98446) in IHC (P)
Immunohistochemical analysis of PCBP2 in paraffin embedded human testis tissue using a PCBP2 polyclonal antibody (Product # PA5-98446) at a dilution of 1:200. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detec... View More
Product Details
PA5-98446
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant Human Poly(rC)-binding protein 2 protein (171-293AA)
Conjugate
Form
Liquid
Concentration
2.69 mg/mL
Purification
Protein G
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.03% ProClin 300
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
The protein encoded by this gene appears to be multifunctional. Along with PCBP-1 and hnRNPK, it is one of the major cellular poly(rC)-binding proteins. The encoded protein contains three K-homologous (KH) domains which may be involved in RNA binding. Together with PCBP-1, this protein also functions as a translational coactivator of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES, promoting poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability. This multiexon structural mRNA is thought to be retrotransposed to generate PCBP-1, an intronless gene with functions similar to that of PCBP2. This gene and PCBP-1 have paralogous genes (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire genes. This gene also has two processed pseudogenes (PCBP2P1 and PCBP2P2). Multiple transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Alpha CP2; Alpha-CP2; Heterogeneous nuclear ribonucleoprotein E2; heterogenous nuclear ribonucleoprotein E2; hnRNP E2; MGC110998; Poly(rC)-binding protein 2; rCbinding protein 2
Gene Aliases: hnRNP-E2; HNRNPE2; HNRPE2; PCBP2
UniProt ID: (Human) Q15366
Entrez Gene ID: (Human) 5094
mRNA splicing, via spliceosome
gene expression
mRNA metabolic process
negative regulation of type I interferon production
viral RNA genome replication
proteasome-mediated ubiquitin-dependent protein catabolic process
innate immune response
negative regulation of defense response to virus
defense response to virus
IRES-dependent viral translational initiation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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