|Tested species reactivity||Bovine, Sheep|
|Published species reactivity||Rat, Bovine, Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic Peptide: M(1) N L E P P K A E I R S A T R(15) C|
|Purification||Ammonium sulfate precipitation|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 4 publications below|
PA1-723 detects phosphodiesterase 6 gamma (PDE6 gamma) from sheep retinal extract and bovine rod outer segment membranes as well as purified bovine PDE6 gamma.
PA1-723 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~10 kDa protein representing PDE6 gamma from sheep retinal extract.
The PA1-723 immunizing peptide corresponds to amino acid residues 1-15 from bovine PDE6 gamma. This peptide (Cat. # PEP-150) is available for use in neutralization and control experiments.
The second messengers cAMP and cGMP are key regulatory molecules that are involved in a wide variety of signal transduction pathways. Levels of cAMP and cGMP are regulated by their rate of synthesis by nucleotide cyclases and by their rate of hydrolysis by cyclic nucleotide phosphodiesterases (PDEs). PDEs form a superfamily of enzymes that catalyze the conversion of 3-prime, 5-prime-cyclic nucleotides to the corresponding nucleoside 5-prime-monophosphates. PDE6 is the effector enzyme in the G protein-mediated signal transduction cascade in the visual system. There are five different subunits consisting of rod and cone specific catalytic subunits: alpha and quote; (Cone), alpha (Rod), and beta (Rod), the inhibitory subunit gamma, and subunit delta of unknown function (which likely interacts with many other proteins besides the PDE6 family). The catalytic core of the PDE6 system is comprised of alpha and quote;/alpha and quote; homodimers in the cone and alpha/beta heterodimers in the rod. The C-terminus of both the catalytic and inhibitory subunits is modified by methylation, myristyolation and prenylation which have been shown to be critical for proper complex assembly and membrane association.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
cGMP-phosphodiesterase 6, transducin and Wnt5a/Frizzled-2-signaling control cGMP and Ca(2+) homeostasis in melanoma cells.
PA1-723 was used in western blot to investigate the importance of PDE6 in the regulation of cGMP metabolism and calcium homeostasis in melanoma cells
|Bazhin AV,Tambor V,Dikov B,Philippov PP,Schadendorf D,Eichmüller SB||Cellular and molecular life sciences : CMLS (67:817)||2010|
The inhibitory gamma subunit of the rod cGMP phosphodiesterase binds the catalytic subunits in an extended linear structure.
PA1-723 was used in western blot to study the interaction between the inhibitory gamma subunit and the catalytic subunit of the rod cGMP phosphodiesterase.
|Guo LW,Muradov H,Hajipour AR,Sievert MK,Artemyev NO,Ruoho AE||The Journal of biological chemistry (281:15412)||2006|
Internalization and recycling of the human prostacyclin receptor is modulated through its isoprenylation-dependent interaction with the delta subunit of cGMP phosphodiesterase 6.
PA1-723 was used in western blot to investigate the role of PDE6 delta in the internalization and recycling of the human prostacyclin receptor.
|Wilson SJ,Smyth EM||The Journal of biological chemistry (281:11780)||2006|
A proline-rich domain in the gamma subunit of phosphodiesterase 6 mediates interaction with SH3-containing proteins.
PA1-723 was used in western blot to characterize the interaction of phosphodiesterase 6 gamma subunit with SH3-containing proteins
|Morin F,Vannier B,Houdart F,Regnacq M,Berges T,Voisin P||Molecular vision (9:449)||2003|