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Invitrogen
PDE6D Polyclonal Antibody
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FIGURE: 1 / 2
PDE6D Antibody (PA1-726) in ICC/IF
Immunofluorescent analysis of PDE6 delta (green) showing staining in the cytoplasm and nucleus of B-3 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a PDE6 delta polyclonal antibody (Product # PA1-726) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated wi... View More
Product Details
PA1-726
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Human,
Sheep
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic Peptides: M(1) S A K D E R A R (D/E) I L R G F K(16) C
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA1-726 detects PrBP/delta from bovine, human and sheep samples.
PA1-726 has been successfully used in Western blot, ICC/IF and immunohistochemistry procedures. By Western blot, this antibody detects a 17 kDa protein representing PrBP/delta from bovine and sheep retinal/optic nerve extracts as well as HeLa cell lysate. Immunohistochemical staining of PrBP/delta in bovine retinal cross-sections using PA1-726 results in staining of retinal outer segments.
PA1-726 immunizing peptides correspond to amino acid residues 1-16 from mouse and human PrBP/delta. This peptide
(Cat. # PEP-203) is available for use in neutralization and control experiments.
Target Information
PrBP/delta, previously known as PDE6/delta, is the effector enzyme in the G protein-mediated signal transduction cascade in the visual system. There are five different subunits consisting of rod and cone specific catalytic subunits: alpha (Cone), alpha (Rod), and beta (Rod), the inhibitory subunit gamma, and subunit delta of unknown function (which likely interacts with many other proteins besides the PDE6 family). PRBP/delta has been implicated in the transport and membrane targeting of prenylated proteins (including PDE6) from their site of synthesis in the inner segment to their final destination in the outer segment of rods and cones (Norton et. al., 2005).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: GMP-PDE delta; PDE6D; phosphodiesterase 6D, cGMP-specific, rod, delta; Protein p17; Retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit delta
Gene Aliases: JBTS22; PDE6D; PDED
UniProt ID: (Bovine) Q95142, (Human) O43924
Entrez Gene ID: (Bovine) 281976, (Human) 5147, (Sheep) 101108369
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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