Abnova

PDGFA Polyclonal Antibody, MaxPab™

View all (24) PDGF-A antibodies

Questions & Answers

Questions & Answers

Product Image Gallery

PDGFA Antibody in Western Blot (WB) — Western Blot analysis of PDGFA expression in transfected 293T cell line (H00005154-T01) by PDGFA MaxPab polyclonal antibody.

Lane 1: PDGFA transfected lysate(24.00 KDa).
Lane 2: Non-transfected lysate.

PDGFA Antibody in Proximity Ligation Assay (PLA) (PLA) — Proximity Ligation Analysis of protein-protein interactions between PDGFA and PDGFRA. HeLa cells were stained with anti-PDGFA rabbit purified polyclonal 1:1200 and anti-PDGFRA mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

PDGFA Antibody in Proximity Ligation Assay (PLA) (PLA) — Proximity Ligation Analysis of protein-protein interactions between PDGFA and PLCG1. Huh7 cells were stained with anti-PDGFA rabbit purified polyclonal 1:1200 and anti-PLCG1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Product Details

H00005154-D01P

Applications	Tested Dilution	Publications
Western Blot (WB)	1-5 µg/mL	-
in situ PLA (PLA)	1:1200	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	PDGFA (NP_002598.4, 1 a.a. approximately 211 a.a) full-length human protein. View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	See Label
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.4
Contains	no preservative
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles

Product Specific Information

Sequence of this protein is as follows: MRTLACLLLL GCGYLAHVLA EEAEIPREVI ERLARSQIHS IRDLQRLLEI DSVGSEDSLD TSLRAHGVHA TKHVPEKRPL PIRRKRSIEE AVPAVCKTRT VIYEIPRSQV DPTSANFLIW PPCVEVKRCT GCCNTSSVKC QPSRVHHRSV KVAKVEYVRK KPKLKEVQVR LEEHLECACA TTSLNPDYRE EDTGRPRESG KKRKRKRLKP T

Target Information

This gene encodes a member of the protein family comprised of both platelet-derived growth factors (PDGF) and vascular endothelial growth factors (VEGF). The encoded preproprotein is proteolytically processed to generate platelet-derived growth factor subunit A, which can homodimerize, or alternatively, heterodimerize with the related platelet-derived growth factor subunit B. These proteins bind and activate PDGF receptor tyrosine kinases, which play a role in a wide range of developmental processes. Alternative splicing results in multiple transcript variants.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?

Let us know so we can reference it here.

Cite this product

Bioinformatics

Protein Aliases: PDGF A-chain; PDGF subunit A; PDGF-1; Platelet-derived growth factor A chain; platelet-derived growth factor A-chain; platelet-derived growth factor alpha chain; Platelet-derived growth factor alpha polypeptide; Platelet-derived growth factor subunit A

Gene Aliases: PDGF-A; PDGF1; PDGFA

UniProt ID: (Human) P04085

Entrez Gene ID: (Human) 5154

Molecular Function: growth factor

MAPK cascade angiogenesis response to hypoxia cell activation hair follicle development positive regulation of mesenchymal cell proliferation platelet degranulation transforming growth factor beta receptor signaling pathway cell-cell signaling positive regulation of cell proliferation response to wounding organ morphogenesis response to inorganic substance negative regulation of phosphatidylinositol biosynthetic process negative regulation of platelet activation regulation of phosphatidylinositol 3-kinase signaling positive regulation of phosphatidylinositol 3-kinase signaling regulation of smooth muscle cell migration cell projection assembly actin cytoskeleton organization extracellular matrix organization positive regulation of cell migration positive regulation of protein autophosphorylation response to estradiol response to retinoic acid regulation of actin cytoskeleton organization positive regulation of metanephric mesenchymal cell migration by platelet-derived growth factor receptor-beta signaling pathway wound healing response to drug positive regulation of MAP kinase activity positive regulation of MAPK cascade positive regulation of GTPase activity skin development positive regulation of DNA replication phosphatidylinositol phosphorylation platelet-derived growth factor receptor signaling pathway phosphatidylinositol-mediated signaling positive regulation of fibroblast proliferation lung alveolus development inner ear development regulation of peptidyl-tyrosine phosphorylation negative chemotaxis positive regulation of cell division positive regulation of protein kinase B signaling regulation of branching involved in salivary gland morphogenesis by epithelial-mesenchymal signaling positive regulation of ERK1 and ERK2 cascade regulation of glomerular mesangial cell proliferation embryonic lung development regulation of DNA biosynthetic process

Process(es)

Ras guanyl-nucleotide exchange factor activity platelet-derived growth factor receptor binding protein binding collagen binding growth factor activity protein homodimerization activity phosphatidylinositol-4,5-bisphosphate 3-kinase activity protein heterodimerization activity platelet-derived growth factor binding