PA3-830 detects human and mouse pdx-1 alpha and shows no cross-reactivity to other pdx isoforms.
PA3-830 has been successfully used in Western blot, immunohistochemical, and immunofluorescence procedures. By Western blot, this antibody detects an ~35 kDa protein.
PA3-830 immunogen is a KLH conjugated synthetic peptide corresponding to amino acid residues 21-35 from human pdx-1 protein. This antibody is generated against the N-terminal portion of the protein, while PA3-831 is C-terminal.
The PA3-830 antibody recognizes both cytoplasmic and nuclear proteins. Formalin-fixed tissues can cause high background, so counterstaining with another antibody which is mainly expressed in the cytoplasm (i.e. insulin) is recommended.
pdx-1 (pancreas duodenum homeobox-1, also known as IPF-1, IDX-1, and STF-1) is a transcription factor that has been shown to activate insulin, glucokinase, IAPP, and somatostatin gene transcription. Significant interest surrounds this proteins glucose-dependent regulation of insulin gene transcription.
Additionally, pdx-1 has been studied for its role in pancreatic development which includes maturation and differentiation of pancreatic precursor cells. Mutations in the pdx-1 gene have been shown to cause pancreatic agenesis, maturity-onset diabetes of the young, and type II diabetes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: DLDBP; E3BP; Glucose-sensitive factor; Insulin promoter factor 1; insulin promoter factor 1, homeodomain transcription factor; Insulin upstream factor 1; IPF-1; Islet/duodenum homeobox 1; Islet/duodenum homeobox-1; IUF-1; Pancreas/duodenum homeobox protein 1; pancreatic and duodenal homeobox gene 1; pancreatic-duodenal homeobox factor 1; PDHX; PDX-1; Pyruvate Dehydrogenase Complex Component-X; Pyruvate Dehydrogenase Complex Lipoyl-Containing Component X; somatostatin transcription factor 1; Somatostatin-transactivating factor 1
Gene Aliases: GSF; IDX-1; IPF-1; IPF1; IUF1; MODY4; PAGEN1; PDX-1; PDX1; STF-1; STF1