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PEMT Polyclonal Antibody
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FIGURE: 1 / 1
PEMT Antibody (PA5150350) in WB
Western blot analysis of PEMT in various lysates. Samples were incubated with PEMT polyclonal antibody (Product # PA5150350) using a dilution of 1:4,000 in 5% NFDM/TBST at 4°C overnight followed by Goat Anti-Rabbit IgG - HRP Secondary Antibody at a dilution of 1:50,000 for 1 hour at room temperature. Lane 1: Mouse liver tissue lysate; Lane 2: Mouse colon tissue lysate; Lane 3: Rat testis tissue lysate. Lysates/proteins at 20 µg/Lane. Predicted band size: 23 kDa; Observed band size: 30 kDa. Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAG... View More
Product Details
PA5150350
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide within mouse PEMT aa 67-93.
Conjugate
Form
Liquid
Concentration
2 mg/mL
Amount
200 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 0.1% BSA, 40% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Positive control: Mouse liver tissue lysate, Mouse colon tissue lysate, Rat testis tissue lysate.
Subcellular location: Endoplasmic reticulum membrane, Mitochondrion membrane.
Target Information
This gene encodes an enzyme which converts phosphatidylethanolamine to phosphatidylcholine by sequential methylation in the liver. The protein localizes to the endoplasmic reticulum and mitochondria-associated membranes. The gene is within the Smith-Magenis syndrome region on chromosome 17. Alternate splicing of this gene results in three transcript variants encoding two different isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: MGC2483; PE N-methyltransferase; PEAMT; Phosphatidylethanolamine N-methyltransferase; Phospholipid methyltransferase; PLMT
Gene Aliases: PEAMT; Pempt; Pempt2; Pemt; PEMT2; PHOMETH
UniProt ID: (Rat) Q08388, (Mouse) Q61907
Entrez Gene ID: (Rat) 25511, (Mouse) 18618
phosphatidyl-N-methylethanolamine N-methyltransferase activity
phosphatidylethanolamine N-methyltransferase activity
N-methyltransferase activity
phosphatidylethanolamine binding
S-adenosylmethionine-dependent methyltransferase activity
phosphatidyl-N-dimethylethanolamine N-methyltransferase activity
methyltransferase activity
transferase activity
methyltransferase
transferase
metabolite interconversion enzyme
phospholipid metabolic process
glycerophospholipid metabolic process
phosphatidylcholine biosynthetic process
response to nutrient
negative regulation of cell proliferation
methylation
response to vitamin
response to drug
response to amino acid
response to ethanol
S-adenosylhomocysteine metabolic process
S-adenosylmethionine metabolic process
positive regulation of lipoprotein metabolic process
lipid metabolic process
phospholipid biosynthetic process
positive regulation of protein targeting to mitochondrion
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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