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Agrisera
PGR5/Proton gradient regulation 5 Polyclonal Antibody
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FIGURE: 1 / 1
PGR5/Proton gradient regulation 5 Antibody (AS16-3985) in WB
Thylakoids were isolated according to Sirpiö et al (2011, Methods Mol Biol. 2011; 775:19-30). 3 µg/µL of total chlorophyll from Arabidopsis thaliana col-0 (1) and Arabidopsis thaliana pgr 5 mutant (Munekage et al. 2002, Cell) (2) were denatured in LaemmLi buffer at room temperature (15 min) were separated on 12% SDS-PAGE mini-gels with 6 M urea and blotted for 1 h to a PVDF membrane using a semi-dry transfe... View More
Product Details
AS16-3985
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Plant
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
KLH-conjugated peptide derived from Arabidopsis thaliana PGR5.
Conjugate
Form
Lyophilized
Storage buffer
whole serum
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana, Picea abies, Pinus sylvestris, Setaria viridis, Solanum lycopersicum
Target Information
PGR5 encodes a small chloroplast thylakoid-membrane protein that localizes to thylakoids where photosystem I electron transport occurs and is genetically and physically linked to the antimycin A-sensitive cyclic electron flow machinery via association with the PGRL1 complex. In Arabidopsis, loss of PGR5 compromises formation of the trans-thylakoid proton gradient (Delta pH) and proton motive force during illumination, indicating that the protein is required for efficient cyclic electron flow around photosystem I and/or its regulation, with consequent effects on ATP supply. Functionally, PGR5 is essential for photoprotection: by supporting Delta pH-dependent energy dissipation (qE component of non-photochemical quenching) and photosynthetic control at the cytochrome b6f complex, it helps prevent over-reduction on the acceptor side of photosystem I and limits photosystem I photoinhibition, particularly under high or fluctuating light.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AtPGR5; proton gradient regulation 5; T20G20.3; T20G20_3
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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