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PHOX2B Antibody (PA5-115754) in ICC/IF
Immunocytochemistry analysis of PHOX2B in A549 cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115754). Secondary staining was applied with mouse anti-beta tubulin (1 hr at 37°, AlexaFluor 594 conjugated goat anti-rabbit IgG (Red), AlexaFluor 488 conjugated goat anti-mouse IgG (Green) and DAPI.
Product Details
PA5-115754
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human PHOX2B(Accession Q99453), corresponding to amino acid residues K152-P202.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2900389
Product Specific Information
Antibody detects endogenous levels of total PHOX2B.
Target Information
The DNA-associated protein encoded by this gene is a member of the paired family of homeobox proteins localized to the nucleus. The protein functions as a transcription factor involved in the development of several major noradrenergic neuron populations and the determination of neurotransmitter phenotype. The gene product is linked to enhancement of second messenger-mediated activation of the dopamine beta-hydroylase, c-fos promoters and several enhancers, including cyclic amp-response element and serum-response element.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: dilated pupils 1; GENA 269; NBPhox; neuroblastoma paired-type homeobox protein; Neuroblastoma Phox; paired mesoderm homeobox 2b; Paired mesoderm homeobox protein 2B; Paired-like homeobox 2B; PHOX2B homeodomain protein
Gene Aliases: Dilp1; NBLST2; NBPhox; PHOX2B; PMX2B
UniProt ID: (Human) Q99453, (Mouse) O35690
Entrez Gene ID: (Human) 8929, (Mouse) 18935
RNA polymerase II regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
DNA binding
sequence-specific DNA binding
homeodomain transcription factor
neuron migration
regulation of respiratory gaseous exchange by neurological system process
noradrenergic neuron differentiation
noradrenergic neuron development
brainstem development
transcription from RNA polymerase II promoter
negative regulation of cell proliferation
glial cell differentiation
regulation of gene expression
positive regulation of G2/M transition of mitotic cell cycle
cell differentiation in hindbrain
medullary reticular formation development
hindbrain tangential cell migration
skeletal muscle cell differentiation
negative regulation of neuron differentiation
positive regulation of neuron differentiation
positive regulation of transcription from RNA polymerase II promoter
autonomic nervous system development
enteric nervous system development
sympathetic nervous system development
parasympathetic nervous system development
inner ear development
efferent axon development in a lateral line nerve
respiratory system development
retrotrapezoid nucleus neuron differentiation
sympathetic ganglion development
negative regulation of cell cycle arrest
dopaminergic neuron differentiation
cellular response to BMP stimulus
neural crest cell migration involved in autonomic nervous system development
regulation of transcription, DNA-templated
multicellular organism development
neuron differentiation
cell development
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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