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Invitrogen
PI3 Polyclonal Antibody
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FIGURE: 1 / 5
PI3 Antibody (PA5-79818) in ICC/IF
Immunocytochemistry analysis of Elafin/Skalp using antiElafin/Skalp antibody (Product # PA5-79818) . Elafin/Skalp was detected in a section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-Elafin/Skalp antibody (Product # PA5-79818) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was ... View More
Product Details
PA5-79818
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived human Elafin/Skalp recombinant protein (Position: A61-Q117).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human placenta tissue. IHC: human tonsil tissue. ICC/IF: A549 cell. Flow: SiHa cell.
Target Information
Peptidase inhibitor 3 (PI3), also known as Elafin, is a gene located on chromosome 20q13.12, encoding a protein that functions as a serine protease inhibitor. Elafin is primarily expressed in epithelial tissues, including skin and mucosal surfaces, where it plays a protective role against excessive proteolytic activity by inhibiting enzymes like neutrophil elastase and proteinase 3. This activity is crucial for maintaining tissue homeostasis and preventing inflammatory damage. PI3 is regulated by pro-inflammatory cytokines and is involved in modulating inflammatory responses and tissue repair processes. Its expression is often elevated in conditions characterized by inflammation, such as chronic obstructive pulmonary disease (COPD), psoriasis, and other inflammatory diseases, reflecting its role in guarding against protease-induced tissue damage. Research into PI3 focuses on its potential therapeutic applications in inflammatory diseases and its utility as a biomarker for assessing inflammation-related pathologies.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Elafin; Elastase-specific inhibitor; ESI; Peptidase inhibitor 3; peptidase inhibitor 3, skin-derived; PI-3; pre-elafin; protease inhibitor 3, skin-derived (SKALP); Protease inhibitor WAP3; SKALP; Skin-derived antileukoproteinase; trappin-2; WAP four-disulfide core domain 14; WAP four-disulfide core domain protein 14
Gene Aliases: cementoin; ESI; PI3; SKALP; WAP3; WFDC14
UniProt ID: (Human) P19957
Entrez Gene ID: (Human) 5266
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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