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Invitrogen

PIAS1 Polyclonal Antibody

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PIAS1 Polyclonal Antibody

Product Details

PA5-87612

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:1,000
-

ELISA (ELISA)

1 µg/mL
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-140 of human PIAS1 (NP_0572501)
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.27 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.09% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2804288

Product Specific Information

Immunogen sequence: MADSAELKQM VMSLRVSELQ VLLGYAGRNK HGRKHELLTK ALHLLKAGCS PAVQMKIKEL YRRRFPQKIM TPADLSIPNV HSSPMPATLS PSTIPQLTYD GHPASSPLLP VSLLGPKHEL ELPHLTSALH PVHPDIKLQK

Positive Samples: HeLa, A-549, Jurkat, HepG2, Mouse kidney; Cellular Location: Nucleus, Nucleus speckle, PML body

Target Information

PIAS1 functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. This protein plays a crucial role in transcriptional coregulation of various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. It functions in testis as a nuclear receptor transcriptional coregulator and may have a role in androgen receptor initiation and maintenance of spermatogenesis. The effects of transcriptional coregulation, transactivation or silencing, may vary depending upon the biological context.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: AR interacting protein; DEAD/H (Asp-Glu-Ala-Asp/His) box binding protein 1; DEAD/H box-binding protein 1; E3 SUMO-protein ligase PIAS1; E3 SUMO-protein transferase PIAS1; GBP; Gu binding protein; Gu-binding protein; nuclear protein that binds to nucleolar RNA helicase Gu protein and to p53; PIAS1/GBP; PIG23; Protein inhibitor of activated STAT protein 1; RING-type E3 ubiquitin transferase PIAS1; RNA helicase II-binding protein; unnamed protein product; zinc finger, MIZ-type containing 3

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Gene Aliases: 2900068C24Rik; DDXBP1; GBP; GU/RH-II; PIAS1; ZMIZ3

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UniProt ID: (Human) O75925, (Mouse) O88907

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Entrez Gene ID: (Human) 8554, (Mouse) 56469, (Rat) 300772

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Function(s)
transcription regulatory region sequence-specific DNA binding DNA binding transcription cofactor activity transcription corepressor activity protein binding zinc ion binding transferase activity SUMO transferase activity enzyme binding protein domain specific binding ubiquitin protein ligase binding metal ion binding SUMO ligase activity DNA-binding transcription factor binding protein C-terminus binding transcription factor binding UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity ligase activity ribosomal S6-glutamic acid ligase activity coenzyme F420-0 gamma-glutamyl ligase activity coenzyme F420-2 alpha-glutamyl ligase activity protein-glycine ligase activity protein-glycine ligase activity, initiating protein-glycine ligase activity, elongating tubulin-glycine ligase activity protein-glutamic acid ligase activity tubulin-glutamic acid ligase activity
Process(es)
G1/S transition of mitotic cell cycle negative regulation of transcription from RNA polymerase II promoter transcription, DNA-templated regulation of transcription, DNA-templated JAK-STAT cascade protein sumoylation positive regulation of proteasomal ubiquitin-dependent protein catabolic process positive regulation of protein sumoylation regulation of cell proliferation negative regulation of apoptotic process fat cell differentiation positive regulation of transcription, DNA-templated positive regulation of smooth muscle cell differentiation protein-DNA complex assembly negative regulation of nucleic acid-templated transcription double-strand break repair via homologous recombination DNA double-strand break processing regulation of transcription from RNA polymerase II promoter cellular response to DNA damage stimulus spermatogenesis visual learning regulation of macromolecule metabolic process positive regulation of protein localization to cell periphery metabolic process
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