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Invitrogen
PIAS1 Polyclonal Antibody
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FIGURE: 1 / 8
PIAS1 Antibody (PA5-99265) in IHC (P)
Immunohistochemistry analysis of PIAS1 in mouse colorectal tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with PIAS1 polyclonal antibody (Product # PA5-99265) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.
Product Details
PA5-99265
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human PIAS1(Accession O75925), corresponding to amino acid residues S4-Q54.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total PIAS1.
Target Information
PIAS1 functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. This protein plays a crucial role in transcriptional coregulation of various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. It functions in testis as a nuclear receptor transcriptional coregulator and may have a role in androgen receptor initiation and maintenance of spermatogenesis. The effects of transcriptional coregulation, transactivation or silencing, may vary depending upon the biological context.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AR interacting protein; DEAD/H (Asp-Glu-Ala-Asp/His) box binding protein 1; DEAD/H box-binding protein 1; E3 SUMO-protein ligase PIAS1; E3 SUMO-protein transferase PIAS1; GBP; Gu binding protein; Gu-binding protein; Protein inhibitor of activated STAT protein 1; protein inhibitor of activated STAT-1; RING-type E3 ubiquitin transferase PIAS1; RNA helicase II-binding protein; zinc finger, MIZ-type containing 3
Gene Aliases: 2900068C24Rik; DDXBP1; GBP; GU/RH-II; PIAS1; ZMIZ3
UniProt ID: (Human) O75925, (Mouse) O88907
Entrez Gene ID: (Human) 8554, (Mouse) 56469, (Rat) 300772
DNA binding
transcription coactivator activity
transcription corepressor activity
protein binding
transcription factor binding
zinc ion binding
ligase activity
SUMO transferase activity
enzyme binding
ubiquitin protein ligase binding
androgen receptor binding
SUMO ligase activity
protein C-terminus binding
UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity
ribosomal S6-glutamic acid ligase activity
protein domain specific binding
coenzyme F420-0 gamma-glutamyl ligase activity
coenzyme F420-2 alpha-glutamyl ligase activity
metal ion binding
protein-glycine ligase activity
protein-glycine ligase activity, initiating
protein-glycine ligase activity, elongating
tubulin-glycine ligase activity
protein-glutamic acid ligase activity
tubulin-glutamic acid ligase activity
G1/S transition of mitotic cell cycle
negative regulation of transcription from RNA polymerase II promoter
transcription, DNA-templated
regulation of transcription, DNA-templated
JAK-STAT cascade
protein sumoylation
positive regulation of proteasomal ubiquitin-dependent protein catabolic process
positive regulation of protein sumoylation
regulation of cell proliferation
negative regulation of apoptotic process
fat cell differentiation
positive regulation of transcription, DNA-templated
positive regulation of smooth muscle cell differentiation
protein-DNA complex assembly
negative regulation of nucleic acid-templated transcription
androgen receptor signaling pathway
regulation of interferon-gamma-mediated signaling pathway
spermatogenesis
metabolic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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