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PIAS3 Polyclonal Antibody
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FIGURE: 1 / 2
PIAS3 Antibody (600-401-DM8) in IHC (P)
Immunohistochemistry of PIAS3 antibody. Tissue: Human breast carcinoma. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: PIAS3 antibody at 5 µg/mL for 1 hr at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: PIAS3 is nuclear and cytoplasmic. Staining: PIAS3 as precipitated brown signal with purple counterst... View More
Product Details
600-401-DM8
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Anti-PIAS3 antibody was prepared from whole rabbit serum produced by repeated immunizations with a 12 amino acid synthetic peptide near the C-terminus of human PIAS3.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
The PIAS (protein inhibitor of activated STAT) proteins play a crucial role as transcriptional coregulators in various cellular pathways, including the STAT, p53 and the steroid hormone signaling pathway. The PIAS protein family includes at least five evolutionarily conserved genes, including PIAS3. The major function of the PIAS proteins is the control of gene transcription and can also act as small ubiquitin-like-modifier (SUMO) E3 ligases. PIAS3 binds specifically to STAT3 following the stimulation of STAT3. Increased expression of PIAS3 has been observed in several human cancers, including lung, breast, and brain tumors, but not in anaplastic lymphoma kinase-positive T/null-cell lymphomas, indicating that PIAS3 plays multiple roles in different tissue and cell types.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: E3 SUMO-protein ligase PIAS3; E3 SUMO-protein transferase PIAS3; KChAP; PIAS3; potassium channel regulatory protein KChAP; Potassium channel-associated protein; Protein inhibitor of activated STAT protein 3; unnamed protein product; zinc finger, MIZ-type containing 5
Gene Aliases: PIAS3; Pias3l; ZMIZ5
UniProt ID: (Human) Q9Y6X2, (Mouse) O54714, (Rat) O70260
Entrez Gene ID: (Human) 10401, (Mouse) 229615, (Rat) 83614
transcription cofactor activity
protein binding
zinc ion binding
transferase activity
SUMO transferase activity
metal ion binding
SUMO ligase activity
protein C-terminus binding
UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity
potassium channel regulator activity
ligase activity
ribosomal S6-glutamic acid ligase activity
enzyme binding
coenzyme F420-0 gamma-glutamyl ligase activity
coenzyme F420-2 alpha-glutamyl ligase activity
protein N-terminus binding
protein-glycine ligase activity
protein-glycine ligase activity, initiating
protein-glycine ligase activity, elongating
tubulin-glycine ligase activity
protein-glutamic acid ligase activity
tubulin-glutamic acid ligase activity
transcription, DNA-templated
regulation of transcription, DNA-templated
positive regulation of gene expression
protein sumoylation
positive regulation of protein sumoylation
positive regulation of membrane potential
response to hormone
negative regulation of transcription from RNA polymerase II promoter
regulation of transcription from RNA polymerase II promoter
negative regulation of gene expression
regulation of tumor necrosis factor-mediated signaling pathway
negative regulation of protein sumoylation
negative regulation of osteoclast differentiation
negative regulation of transcription, DNA-templated
regulation of macromolecule metabolic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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