Bioss

PIBF Polyclonal Antibody

View all (12) PIBF1 antibodies

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Datasheet

Protocols

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PIBF Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Paraformaldehyde-fixed, paraffin embedded Rat Testis; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (BSA or normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-PIBF Polyclonal Antibody, Unconjugated (bs-0420R ) at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining.

PIBF Antibody in Western Blot (WB) — Lane 1: Testis (Mouse) lysates; Lane 2: Testis (Rat) lysates probed with PIBF Polyclonal Antibody, Unconjugated (bs-0420R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.

Product Details

BS-0420R

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:500	-
Immunohistochemistry (Frozen) (IHC (F))	1:100-1:500	-
Immunocytochemistry (ICC/IF)	1:50-1:200	-
ELISA (ELISA)	1:500-1:1,000	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	KLH conjugated synthetic peptide derived from human PIBF, amino acids 551-650. View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Protein A
Storage buffer	PBS with 1% BSA, 50% glycerol
Contains	0.09% sodium azide
Storage conditions	-20°C

Target Information

Mediator of progesterone that by acting on the phospholipase A2 enzyme interferes with arachidonic acid metabolism, induces a Th2 biased immune response, and by controlling NK activity exerts an anti-abortive effect.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Centrosomal protein of 90 kDa; CEP90; PIBF; progesterone immunomodulatory binding factor 1; progesterone-induced blocking factor 1; Progesterone-induced-blocking factor 1; RP11-505F3.1

Gene Aliases: C13orf24; CEP90; PIBF; PIBF1; RGD1305077

UniProt ID: (Human) Q8WXW3

Entrez Gene ID: (Human) 10464, (Mouse) 52023, (Rat) 306104

Function(s)

biological_process immune system process mitotic metaphase plate congression activation of JAK1 kinase activity negative regulation of prostaglandin biosynthetic process negative regulation of interleukin-12 production positive regulation of interleukin-10 production negative regulation of natural killer cell activation nonmotile primary cilium assembly negative regulation of tyrosine phosphorylation of Stat4 protein positive regulation of tyrosine phosphorylation of Stat6 protein protein localization to centrosome mitotic spindle assembly

Process(es)

interleukin-4 receptor binding protein binding molecular_function

PIBF Polyclonal Antibody

Product Details

BS-0420R

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Target Information

References (0)

Bioinformatics

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