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Agrisera
PIF3/Phytochrome interacting factor 3 Polyclonal Antibody
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FIGURE: 1 / 1
PIF3/Phytochrome interacting factor 3 Antibody (AS16-3954) in WB
Total protein from Arabidopsis thaliana (10-20 µg) of 4-d-old dark grown Col-0, pifsingle, triple and quadruple mutants were extracted with the buffer described in Qiu et al. 2015 and denatured at 95°C for 5 min. were separated on 8% Bis-Tris SDS-PAGE, and subsequently blotted to Nitrocellulose membrane using wet tank transfer. Blots were blocked with TBS containing 2% non-fat milk at room temperature for 1... View More
Product Details
AS16-3954
Product Specifications
Species Reactivity
Plant
Host/Isotype
Goat
Class
Polyclonal
Type
Antibody
Immunogen
KLH-conjugated peptide, derived from Arabidopsis thaliana PIF3.
Conjugate
Form
Lyophilized
Amount
50 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles,Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana
Target Information
PIF3 is a nuclear-encoded transcription factor that accumulates in the nucleus and functions as a direct signaling partner of photoactivated phytochromes to couple light perception to transcriptional reprogramming during seedling development. The encoded protein is a basic helix-loop-helix regulator with a C-terminal bHLH DNA-binding/dimerization domain and an N-terminal PAS (Per-Arnt-Sim-like) region that supports protein-protein interactions, consistent with its role as a phytochrome-interacting factor. Functionally, PIF3 binds G-box-type cis elements in promoters of light-regulated genes and acts as a key modulator of photomorphogenesis and chloroplast biogenesis, contributing to rapid light-induced expression of nuclear genes needed for establishment of the photosynthetic apparatus at the dark-to-light transition while also repressing chlorophyll biosynthesis/photosynthesis programs in darkness through recruitment of chromatin repressors such as the histone deacetylase HDA15.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: F14J9.19; F14J9_19; PAP3; PHOTOCURRENT 1; PHYTOCHROME INTERACTING FACTOR 3; PHYTOCHROME-ASSOCIATED PROTEIN 3; POC1; purple acid phosphatase 3
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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