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Agrisera
PIF5/Phytochrome interacting factor 5 Polyclonal Antibody
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FIGURE: 1 / 2
PIF5/Phytochrome interacting factor 5 Antibody (AS12-2112) in WB
10 µg of total protein from 4 day old dark grown Arabidopsis thaliana seedlings extracted with hot buffer (Shen et al., Plant Cell. 2008; 20(6): 1,586-602) were separated on 8% SDS-PAGE and blotted 1 h to PVDF using semi-dry. Blots were blocked with the blocking buffer from Protein Detector Lumi GLO western blotting kit (KPL, Cat: 54-12-50) for 1 h at room temperature (RT) with agitation. Blot was incubated... View More
Product Details
AS12-2112
Product Specifications
Species Reactivity
Plant
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana PIF5.
Conjugate
Form
Lyophilized
Amount
50 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana
Target Information
PIF5 encodes a plant nuclear transcription factor that accumulates in darkness and functions as a central node in phytochrome-dependent light signaling in Arabidopsis. The protein is a basic helix-loop-helix DNA-binding factor with a C-terminal bHLH region that mediates sequence-specific binding to G-box class cis-elements in target promoters, and it carries an N-terminal active phytochrome B binding (APB) motif that confers selective interaction with the photoactivated phyB photoreceptor and supports rapid red-light-triggered phosphorylation and proteasome-dependent turnover. Functionally, PIF5 helps couple environmental light and photoperiod cues to transcriptional reprogramming that shapes early seedling development and growth, and it directly promotes dark-induced leaf senescence and chlorophyll catabolism by activating senescence-associated regulators and chlorophyll breakdown genes (for example via G-box-dependent control of ORE1 and SGR/NYC1 pathway components).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: A-PUT2; AT3G59060; AtbHLH65; BHLH065; bHLHb1; F17J16.110; PIL6
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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