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Invitrogen
PIWIL2 Polyclonal Antibody
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FIGURE: 1 / 6
PIWIL2 Antibody (PA5-102687) in IHC (P)
Immunohistochemistry analysis of PIWIL2 in mouse testis tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with PIWIL2 polyclonal antibody (Product # PA5-102687) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.
Product Details
PA5-102687
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human PIWIL2(Accession Q8TC59), corresponding to amino acid residues C791-E841.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total PIWIL2.
Target Information
The piwi gene of Drosophila belongs to a novel class of evolutionarily conserved genes (the piwi or Argonaute family). The Piwi family is required for germ and stem cell development in invertebrates, and two Piwi members, MIWI and MILI (Miwi like, Piwil2, Piwi-like protein 2), are essential for spermatogenesis in mouse. MIWI and MILI partner with Piwi-interacting RNAs (piRNAs) which are 26-31 nucleotides in length clearly distinct from the 21-23 nucleotides of microRNAs (miRNAs) or short interfering RNAs (siRNAs). A search for murine small RNAs that might program Piwi proteins for transposon suppression revealed developmentally regulated piRNA loci, some of which resemble transposon master control loci of Drosophila. MILI might be programmed by distinct piRNA populations at different stages of germ cell development, suggesting MILI plays an important role in meiotic prophase progression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 80kDa PIWIL2 short isoform; Cancer/testis antigen 80; CT80; Miwi like; Miwi likw; piwi like homolog 2; PIWI-L2; piwi-like 2; piwi-like homolog 2; Piwi-like protein 2; piwi-like RNA-mediated gene silencing 2; piwil2-like protein; testicular tissue protein Li 141
Gene Aliases: CT80; HILI; Mili; PIWIL1L; PIWIL2
UniProt ID: (Human) Q9NW28, (Mouse) Q8CDG1
Entrez Gene ID: (Human) 55124, (Rat) 306011, (Mouse) 57746
RNA 5'-end processing
multicellular organism development
spermatogenesis
germ-line stem cell population maintenance
gene silencing by RNA
piRNA metabolic process
DNA methylation involved in gamete generation
positive regulation of translation
oogenesis
meiotic cell cycle
positive regulation of meiosis I
positive regulation of histone H3-K14 acetylation
positive regulation of histone H3-K9 acetylation
pachytene
regulation of translation
cell differentiation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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