Invitrogen

PLD2 Polyclonal Antibody

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PLD2 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of PLD2 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C) incubated with PLD2 polyclonal antibody (Product # PA5-104015) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 at a dilution of 1:600.

PLD2 Antibody in Western Blot (WB) — Western blot analysis of PLD2 in HUVEC cell lysate. Samples were incubated with PLD2 polyclonal antibody (Product # PA5-104015).

Product Details

PA5-104015

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000-1:3,000	-
Immunocytochemistry (ICC/IF)	1:100-1:500	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	A synthesized peptide derived from human PLD2(Accession O14939), corresponding to amino acid residues A495-R524. View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.4, with 50% glycerol, 150mM NaCl
Contains	0.02% sodium azide
Storage conditions	-20°C
RRID	AB_2853346

Target Information

PLD2 is a peripheral membrane protein belonging to phospholipase D family with a PH domain, two PLD phosphodiesterase domains and a PX (phox homology) domain that plays a regulatory rather than catalytic role. PLD2 catalyzes the hydrolysis of phosphatidylcholine (PC) to produce phosphatidic acid and choline. PLD2 may function in regulated secretion, signal-induced cytoskeletal regulation and/or endocytosis, transcriptional regulation, and cell cycle control. It is usually stimulated by phosphatidylinositol 4,5-bisphosphate (PIP2) and activated by the ADP-ribosylation factor-1 (ARF-1). PLD2 is activated by agonist stimulation of both tyrosine kinase and G protein-coupled receptors and is also known to interact with EGFR and PIP5K1A. PLD2, localized to plasma membrane caveolae, is ubiquitously expressed in most tissues.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Choline phosphatase 2; hPLD2; Phosphatidylcholine-hydrolyzing phospholipase D2; phospholipase D gene 2; Phospholipase D2; PLD 2; PLD1C; rPLD2

Gene Aliases: PLD1C; PLD2; Pldc

UniProt ID: (Human) I3L2C9, (Mouse) P97813, (Rat) P70498

Entrez Gene ID: (Human) 5338, (Mouse) 18806, (Rat) 25097

Molecular Function: phospholipase