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Invitrogen
PLK4 Polyclonal Antibody
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FIGURE: 1 / 3
PLK4 Antibody (PA5-29373) in ICC/IF
Immunocytochemistry (Confocal immunofluorescence) of PLK4 in methanol-fixed HeLa. Samples were incubated with PLK4 polyclonal antibody (Product # PA5-29373) using a dilution of 1:500 (green). Alpha-tubulin filaments were labeled with alpha Tubulin monoclonal antibody (Product # MA1-25054) (Red) at 1:2,000.
Product Details
PA5-29373
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human PLK4. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.19 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol, 1% BSA
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: PLK4-transfected 293T.
Predicted reactivity: Bovine (87%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
PLK4 or polo-like kinase 4 is a member of the polo family of serine/threonine protein kinases which localizes to centrioles, complex microtubule-based structures found in centrosomes, and regulates centriole duplication during the cell cycle. The overexpression of PLK4 triggered the simultaneous formation of multiple procentrioles around each preexisting centriole which resulting in centriole amplification and thus, Plk4-induced centriole biogenesis in human cells. The reduced Plk4 gene dosage increases the probability of mitotic errors and cancer development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: PLK-4; Polo-like kinase 4; Serine/threonine-protein kinase 18; Serine/threonine-protein kinase PLK4; Serine/threonine-protein kinase Sak; Snk akin kinase; unnamed protein product
Gene Aliases: MCCRP2; PLK4; SAK; STK18
UniProt ID: (Human) O00444
Entrez Gene ID: (Human) 10733
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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