|ELISA (ELISA)||Assay dependent|
|Immunohistochemistry (Frozen) (IHC (F))||1:20-1:200|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20-1:200|
|Western Blot (WB)||1:1,000|
|Immunohistochemistry (Paraffin) (IHC (P))||See 1 publications below|
|Miscellaneous PubMed (MISC)||See 1 publications below|
|Western Blot (WB)||See 4 publications below|
|Immunohistochemistry (IHC)||See 2 publications below|
|Immunocytochemistry (ICC)||See 3 publications below|
|Immunoprecipitation (IP)||See 1 publications below|
|Tested Species reactivity||Human, Pig, Rat|
|Published species reactivity||Rat , Pig , Human , Mouse , Chicken|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic Peptide: T(5) N S D F Y S K N Q R N E S S(19)|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA1-915 detects calcium pump of the plasma membrane 2 (PMCA2 ATPase) from human and rat and porcine tissues and cells.
PA1-915 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~127 kDa protein and an ~133 kDa protein representing PMCA2a and PMCA2b ATPase, respectively, from rat brain microsomal fractions.
PA1-915 immunizing peptide corresponds to amino acid residues 5-19 from human PMCA2 ATPase protein. This sequence is completely conserved between human and rat PMCA2 ATPase.
The calcium pump of the plasma membrane, termed PMCA ATPase, pumps calcium from the cytosol to the extracellular space. This membrane-bound enzyme is related to a number of other ATPases including the SERCA ATPase and the sodium/K+ pump.
There are four different genes encoding PMCA ATPase and studies have revealed 20 isoforms of the pump generated by alternate splicing of the primary gene products. mRNA distribution studies show that gene products 1 and 4 are transcribed in most tissues, however, products 2 and 3 are more tissue specific. Transcription of the splicing variants has also been found to be tissue specific. In the pancreas, where insulin secretion is calcium dependent, the beta cells only express the 4b isoform, however the alpha and gamma cells express both 4a and 4b isoforms. Studies have also shown that different splice variants have different affinities for calcium and calmodulin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: ATPase isoform 2 Na+K+ transporting beta polypeptide 2; ATPase isoform 2, Na+K+ transporting, beta polypeptide 2; ATPase, Ca++ transporting, plasma membrane 2; plasma membrane Ca(2+)-ATPase; plasma membrane Ca++-ATPase; plasma membrane Ca2+ pump 2; plasma membrane calcium ATPase; Plasma membrane calcium ATPase isoform 2; plasma membrane calcium pump; Plasma Membrane Calcium Pump ATPase 2; Plasma membrane calcium pump isoform 2; Plasma membrane calcium-transporting ATPase 2; PMCA2
Gene Aliases: ATP2B2; PMCA2; PMCA2a; PMCA2i
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.